Paxillin Antibody | Rockland Immunochemicals 200-301-P44 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

Paxillin Antibody

catalog :

200-301-P44

quantity :

100 µg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

PAX-14

reactivity :

human, mouse, rat, chicken

application :

western blot, ELISA, immunohistochemistry

more info or order :

Rockland Immunochemicals product webpage

product information

Catalog Number :

200-301-P44

Name :

Anti-Paxillin (MOUSE) Antibody - 200-301-P44

Display Name :

Paxillin Antibody

Application Note :

Anti-Paxillin is useful for Immunohistochemistry and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~64.5 kDa corresponding to the appropriate cell lysate or extract.

Buffer :

1.2% sodium acetate

Clonality :

Monoclonal

Clone ID :

PAX-14

Concentration Value :

1.0 mg/mL

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

(None)

Size :

100 µg

Default Unit :

µg

ELISA Dilution :

1:20,000

Immunohistochemistry Dilution :

1:100-1:500

Western Blot Dilution :

1-2ug/ml

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG1

Gene Name :

PXN

Host Animal :

Mouse

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

Paxillin antibody was produced in mice by repeated immunizations with recombinant protein corresponding to the C-terminal part of chicken paxillin (amino acids 305-559).

Packing Type :

Ambient

Physical State :

Lyophilized

Preservative :

0.01% (w/v) Sodium Azide

Purity and Specificity :

Anti-Paxillin antibody was purified from mouse ascites by protein A chromatography. This product reacts with human, mouse, rat, and chicken. Cross reactivity with Paxillin from other sources has not been determined.

Reconstitution Buffer :

Neutral PBS

Reconstitution Volume :

1.0 mL

Species Reactivity :

Human, mouse, rat, chicken

Stabilizer :

2mg BSA

Storage :

Store vial at 4° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Subclass :

IgG1

Synonyms :

Paxillin antibody, PXN

Target Species :

Chicken

Background :

The paxillin gene can be alternatively spliced to include 1 of 2 alternative exons, generating beta and gamma isoforms. Paxillin is a 68-kDa focal adhesion protein that is phosphorylated on tyrosine residues in fibroblasts in response to transformation by v-src, treatment with platelet-derived growth factor, or cross-linking of integrins. The 68-kD protein (paxillin) is a cytoskeletal component that localizes to the focal adhesions at the ends of actin stress fibers in chicken embryo fibroblasts. It is also present in the focal adhesions of Madin-Darby bovine kidney (MDBK) epithelial cells but is absent, like talin, from the cell-cell adherens junctions of these cells. This antibody is suitable for researchers interested in growth factors and signal trasduction.

Immunogen Type :

Recombinant Protein

Low Endotoxin :

Sample Size :

Target Domain of Reactivity :

C-Terminus

Application Text :

ELISA,Immunohistochemistry,Western Blot,

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

UniProt :

P49023

NCBI :

NP_001074324.1

Primary Image Name :

Western Blot of Mouse Anti-Paxillin antibody.

more info or order :

Rockland Immunochemicals product webpage