Nitric Oxide Synthase (NOS1) Antibody | Rockland Immunochemicals 200-301-P43 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

Nitric Oxide Synthase (NOS1) Antibody

catalog :

200-301-P43

quantity :

100 µg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

reactivity :

human, rat

application :

western blot, ELISA

more info or order :

Rockland Immunochemicals product webpage

product information

Catalog Number :

200-301-P43

Name :

Anti-Nitric Oxide Synthase (NOS1) (MOUSE) Antibody - 200-301-P43

Display Name :

Nitric Oxide Synthase (NOS1) Antibody

Application Note :

Anti-NOS1 is useful for Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~161 kDa corresponding to the appropriate cell lysate or extract.

Buffer :

1.2% sodium acetate

Clonality :

Monoclonal

Clone ID :

Concentration Value :

1.0 mg/mL

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

(None)

Size :

100 µg

Default Unit :

µg

ELISA Dilution :

1:5000

Western Blot Dilution :

0.5ug/ml

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG1

Gene Name :

NOS1

Host Animal :

Mouse

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

Nitric Oxide Synthase (NOS1) antibody was produced in mice by repeated immunizations with recombinant protein corresponding to neuronal NOS fragment (amino acids 1-181) from rat brain.

Packing Type :

Ambient

Physical State :

Lyophilized

Preservative :

0.01% (w/v) Sodium Azide

Purity and Specificity :

Anti-NOS1 antibody was purified from mouse ascites by protein A chromatography. This product reacts with human, rat, goat, and pig. Cross reactivity with NOS1 from other sources has not been determined.

Reconstitution Buffer :

Neutral PBS

Reconstitution Volume :

1.0 mL

Species Reactivity :

Human, rat, goat, pig

Stabilizer :

2mg BSA

Storage :

Store vial at 4° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Subclass :

IgG1

Synonyms :

NOS, bNOS, nNOS, IHPS1, N-NOS, NC-NOS, Neuronal NOS, Peptidyl-cysteine S-nitrosylase NOS1

Target Species :

Human

Background :

Nitric Oxide Synthase 1(NOS1,neuronal NOS,nNOS1) is a messenger molecule, mediating the effect of endothelium-derived relaxing factor in blood vessels and the cytotoxic actions of macrophages, and playing a part in neuronal communication in the brain. It may be involved in neuronal cell death and damage in neurological illness. nNOS1 localized to the 12q24.2 region of human chromosome 12. It splice variant, expressed in testis, that encodes an NH2-terminal truncated protein of 1098 amino acids. nNOS cDNA clones were shown to contain different 5' terminal exons spliced to a common exon 2. Genomic cloning and sequence analysis demonstrate that the unique exons are positioned within 300 bp of each other but separated from exon 2 by an intron that is at least 20 kb in length. The neuronal isoform of nitric oxide synthase is highly expressed in mammalian skeletal muscle, it suggested a specific role for NOS1 in the local metabolic inhibition of alpha-adrenergic vasoconstriction in active skeletal muscle. The novel gaseous neuromediator nitric oxide is thought to play an important role in development and plasticity. Despite this, gene-knockout mice lacking neuronal (Type I) nitric oxide synthase exhibit relatively normal brain development and behavior. This antibody is suitable for researchers interested in apoptosis research, cell death, and TNF pathways.

Immunogen Type :

Recombinant Protein

Low Endotoxin :

Sample Size :

Application Text :

ELISA,Western Blot,

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

UniProt :

P29475

NCBI :

NP_000611.1

more info or order :

Rockland Immunochemicals product webpage