CDC6 Antibody | Rockland Immunochemicals 200-301-P29 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

CDC6 Antibody

catalog :

200-301-P29

quantity :

100 µg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

IMD-6

reactivity :

human

application :

western blot, ELISA

more info or order :

Rockland Immunochemicals product webpage

product information

Catalog Number :

200-301-P29

Name :

Anti-CDC6 (MOUSE) Antibody - 200-301-P29

Display Name :

CDC6 Antibody

Application Note :

Anti-CDC6 is useful for Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~62.7 kDa corresponding to the appropriate cell lysate or extract.

Buffer :

1.2% sodium acetate

Clonality :

Monoclonal

Clone ID :

IMD-6

Concentration Value :

1.0 mg/mL

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

(None)

Size :

100 µg

Default Unit :

µg

ELISA Dilution :

1:20,000

Western Blot Dilution :

1-2µg/ml

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG1

Gene Name :

CDC6

Host Animal :

Mouse

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

CDC6 antibody was produced in mice by repeated immunizations with a recombinant protein corresponding to human Cdc6.

Packing Type :

Ambient

Physical State :

Lyophilized

Preservative :

0.01% (w/v) Sodium Azide

Purity and Specificity :

Anti-CDC6 antibody was purified from mouse ascites by protein A chromatography. This product reacts with human. Cross reactivity with CDC6 from other sources has not been determined.

Reconstitution Buffer :

Neutral PBS

Reconstitution Volume :

1.0 mL

Species Reactivity :

Human

Stabilizer :

2mg BSA

Storage :

Store vial at 4° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Subclass :

IgG1

Synonyms :

CDC18L, HsCdc18, p62(cdc6), CELL DIVISION CYCLE 18, S. POMBE, HOMOLOG-LIKE, CDC6 (cell division cycle 6, S. cerevisiae) homolog, CELL CYCLE CONTROLLER CDC6, CDC6-related protein, Cdc18-related protein

Target Species :

Human

Background :

In yeasts, Cdc6 (Saccharomyces cerevisiae) and Cdc18 (Schizosaccharomyces pombe) associate with the origin recognition complex (ORC) proteins to render cells competent for DNA replication. Cdc6 is overexpressed in human cancers, where it has a critical regulatory role in addition to DNA replication. Yan et al. (1998) showed that Cdc6 is expressed selectively in proliferating but not quiescent mammalian cells, both in culture and within tissues in intact animals. During the transition from a growth-arrested to a proliferative state, transcription of mammalian Cdc6 is regulated by E2F proteins, as revealed by a functional analysis of the human Cdc6 promoter and by the ability of exogenously expressed E2F proteins to stimulate the endogenous Cdc6 gene. They conclude that expression of human Cdc6 is regulated in response to mitogenic signals though transcriptional control mechanisms involving E2F proteins, and that Cdc6 is required for initiation of DNA replication in mammalian cells. This antibody is suitable for researchers interested in cancer research and Cell Cycle research.

Immunogen Type :

Recombinant Protein

Low Endotoxin :

Sample Size :

Application Text :

ELISA,Western Blot,

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

UniProt :

Q99741

NCBI :

NP_001245.1

more info or order :

Rockland Immunochemicals product webpage