product summary
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company name :
Rockland Immunochemicals
product type :
antibody
product name :
CDC25C Antibody
catalog :
200-301-P28
quantity :
100 µg
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
IMD-25
reactivity :
human
application :
western blot, ELISA
product information
Catalog Number :
200-301-P28
Name :
Anti-CDC25C (MOUSE) Antibody - 200-301-P28
Display Name :
CDC25C Antibody
Application Note :
Anti-CDC25C is useful for Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~53.4 kDa corresponding to the appropriate cell lysate or extract.
Buffer :
1.2% sodium acetate
Clonality :
Monoclonal
Clone ID :
IMD-25
Concentration Value :
1.0 mg/mL
Concentration Definition :
by UV absorbance at 280 nm
Conjugation :
(None)
Size :
100 µg
Default Unit :
µg
ELISA Dilution :
1:20,000
Western Blot Dilution :
1-2µg/ml
Expiration :
Expiration date is one (1) year from date of opening.
Format :
IgG1
Gene Name :
CDC25C
Host Animal :
Mouse
General Disclaimer Note :
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Immunogen :
CDC25C antibody was produced in mice by repeated immunizations with a recombinant protein corresponding to human Cdc25c.
Packing Type :
Ambient
Physical State :
Lyophilized
Preservative :
0.01% (w/v) Sodium Azide
Purity and Specificity :
Anti-CDC25C antibody was purified from mouse ascites by protein A chromatography.  This product reacts with human. Cross reactivity with CDC25C from other sources has not been determined.
Reconstitution Buffer :
Neutral PBS
Reconstitution Volume :
1.0 mL
Species Reactivity :
Human
Stabilizer :
2mg BSA
Storage :
Store vial at 4° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Subclass :
IgG1
Synonyms :
CDC25, PPP1R60, MITOSIS INDUCER CDC25, cell division cycle 25C, protein phosphatase 1, regulatory subunit 60, Dual specificity phosphatase Cdc25C
Target Species :
Human
Background :
Cell division sycle(CDC25) gene product is a protein-tyrosine phosphatase, activates a partially purified p34(cdc2)/cyclin B complex. The cdc25 protein also shares homology with a protein phosphatase with activity against both tyrosine and serine (and thus probably threonine) phosphate residues. CDC25C is expressed predominantly in G2 phase in healer cells. The human gene encodes a protein with a predicted molecular mass of 53,000 daltons whose C-terminal domain shares about 37% sequence identity with the fission yeast cdc25 mitotic inducer. CDC25C gene is mapped to 5q31. This antibody is suitable for researchers interested in cancer research, autoimmune disease, cardiovascular disease, neurodegenerative disease, MAK Kinase Signaling, AKT Signaling, GPCR Signaling, Cell Cycle research, and nuclear receptors and transcription factors.
Immunogen Type :
Recombinant Protein
Low Endotoxin :
No
Sample Size :
No
Application Text :
ELISA,Western Blot,
Category :
Primary Antibodies
Conjugation Name :
Unconjugated
UniProt :
P30307
NCBI :
NP_001274511.1
company information
Rockland Immunochemicals
321 Jones Blvd
Pottstown, PA 19464
tech@rockland.com
https://www.rockland.com/
484-791-3823
headquarters: USA
Rockland Immunochemicals, Inc. produces Phosho-Site Specific Antibodies and Antibody based tools for basic, applied and clinical research. Our laboratory is located west of Philadelphia, Pennsylvania, USA. The technology base of the organization is the experience of its staff scientists in the production and purification of a wide range of antibodies through monoclonal & polyclonal antibody techniques utilizing in vitro and in vivo methods. Rockland's antibodies are suited for individuals performing Western Blotting, ELISA, Immunohistochemistry, Fluorescent Microscopy, High Content Screening and diagnostic kit production. Primary antibodies include Akt Pathway, Apolipoproteins, Apoptosis/Cell Cycle, Cytokines, Cell Signaling, Enzymes, Extracellular Matrix, Transcription Factors (NFkB) and Ubiquitin.