SARS-CoV-2 Spike Protein (S2) Antibody | Rockland Immunochemicals 200-301-MU3 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

SARS-CoV-2 Spike Protein (S2) Antibody

catalog :

200-301-MU3

quantity :

100

clonality :

polyclonal

host :

mouse

conjugate :

nonconjugated

clone name :

4D1.H6.D6.C12.G3

reactivity :

SARS-CoV-2

application :

western blot, ELISA

more info or order :

Rockland Immunochemicals product webpage

image

image 1 :

ELISA results of Mouse Anti-SARS CoV-2 Spike Protein (S2) Antibody. Each well was coated in duplicate with 0.5µg of rec SARS CoV-2 Whole Spike protein [RED line], rec SARS CoV-2 Spike (S1) [GREEN line], rec SARS CoV-2 Spike (S1) RBD protein [BLUE line], and rec SARS CoV-2 Spike (S2) glycoprotein [PURPLE line]. The working dilution for SARS-CoV-2 whole spike is 1:190,000 and SARS CoV-2 Spike (S2) glycoprotein is 1:120,000. The starting dilution of antibody was 0.55μg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Rabbit Anti-Mouse IgG HRP conjugated (p/n 610-403-C46) at 1:8000 and TMB substrate (p/n TMBE-1000). SARS CoV-2 Spike (S2) EC50: 8ng/mL; SARS CoV-2 Whole Spike EC50: 5ng/mL.

product information

Catalog Number :

200-301-MU3

Name :

Anti-SARS-CoV-2 Spike Protein (S2) (MOUSE) Antibody - 200-301-MU3

Display Name :

SARS-CoV-2 Spike Protein (S2) Antibody

Application Note :

Anti-SARS-CoV-2 Spike Protein (S2) Antibody has been tested for use in ELISA and shows reactivity to SARS COV-2 Whole Spike and COV-2 Spike (S2) protein. Specific conditions for reactivity should be optimized by the end user.

Buffer :

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Polyclonal

Clone ID :

4D1.H6.D6.C12.G3

Concentration Value :

1.12 mg/mL

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

(None)

Size :

100

Default Unit :

µg

ELISA Dilution :

1:70,000-1:150,000

Western Blot Dilution :

User Optimized

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG

Host Animal :

Mouse

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

This protein A purified antibody was produced by repeated immunizations with a purified SARS-CoV-2 S protein with His-tag, derived from the transfected human HEK293 cells.

Packing Type :

Dry Ice

Physical State :

Liquid (sterile filtered)

Preservative :

0.01% (w/v) Sodium Azide

Purity and Specificity :

Anti-SARS-CoV-2 Spike S2 Antibody is directed against SARS Coronavirus 2 Spike (S2) protein. The product was purified from roller bottle culture by protein A chromatography. BLAST analysis was used to suggest reactivity with related Coronavirus proteins. No reactivity was seen against SARS CoV-2 Spike S1 subunit, SARS CoV-2 Spike S1 RBD, SARS CoV-2 loop, SARS CoV-2 (N), MERS CoV. Cross reactivity with homologues from other sources has not been determined.

Species Reactivity :

SARS Coronavirus 2 Spike S2

Storage :

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Subclass :

IgG1 kappa

Synonyms :

mouse anti-SARS CoV 2 Spike S2 Protein Antibody, Spike glycoprotein antibody, SARS CoV2 antibody, 2019-nCoV, COVID-19, S glycoprotein, Severe acute respiratory syndrome antibody, Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) (SARS-CoV-2)

Target Species :

SARS

Background :

SARS-CoV-2 (Severe acute respiratory syndrome coronavirus 2) the virus that causes the disease COVID-19, is related to SARS-CoV, MERS-CoV, and other betacoronaviruses (HKU1, NL63, OC43 and 229E). SARS-CoV-2 is an enveloped positive strand RNA virus that consists of several structural proteins including spike (S) protein, envelope (E) protein, membrane (M) protein and nucleocapsid (N) protein; and other non-structural proteins (NSPs). Spike proteins located on the surface of the virus, have a high affinity for binding to human receptor ACE2 (angiotensin-converting enzyme 2) within respiratory epithelial which serves as an entry mechanism for infection. As such, the S protein may be vital to blocking viral infection and vaccine development. Anti-SARS-CoV-2 Spike Protein (S2) Antibody is useful for researchers interested in diagnostics, vaccines, and viral research.

Immunogen Type :

Recombinant Protein

Low Endotoxin :

Sample Size :

Application Text :

ELISA,Western Blot,

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

Primary Image Name :

ELISA results of Mouse Anti-SARS CoV-2 Spike Protein (S2) Antibody

New Product :

Yes

more info or order :

Rockland Immunochemicals product webpage