product summary
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company name :
Rockland Immunochemicals
product type :
antibody
product name :
TLR9 Antibody
catalog :
200-301-I31
quantity :
100 µg
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
26C593.2
reactivity :
human, mouse, rat
application :
western blot, immunohistochemistry, flow cytometry
product information
Catalog Number :
200-301-I31
Name :
Anti-TLR9 (MOUSE) Monoclonal Antibody - 200-301-I31
Display Name :
TLR9 Antibody
Application Note :
Anti-TLR9 Antibody is suitable for use in CM, Flow (CS), Flow (I), IF/ICC, IHC (frozen), IHC (p) and WB. Expect a band approximately 113kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.
Buffer :
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Clonality :
Monoclonal
Clone ID :
26C593.2
Concentration Value :
1.0 mg/mL
Concentration Definition :
by UV absorbance at 280 nm
Conjugation :
(None)
Size :
100 µg
Default Unit :
µg
Flow Cytometry Dilution :
0.1-2 µg/10^6 cells
Immunohistochemistry Dilution :
10-20 µg/mL (frozen)
Western Blot Dilution :
1-5 µg/mL
Expiration :
Expiration date is one (1) year from date of opening.
Format :
IgG1
Gene Name :
TLR9
Host Animal :
Mouse
General Disclaimer Note :
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Immunogen :
TLR9 Antibody was produced in mice prepared repeated immunizations with amino acids corresponding to an internal sequence of human TLR9 isoform A protein.
Packing Type :
Dry Ice
Physical State :
Liquid
Preservative :
0.05% (w/v) Sodium Azide
Purity and Specificity :
Anti-TLR9 Antibody was purified by Protein G chromatography. A BLAST analysis was used to suggest cross-reactivity with Anti-TLR9 from monkey based on 100% homology with the immunizing sequence. Cross-reactivity with Anti-TLR9 from other sources has not been determined.
Species Reactivity :
Human, Mouse, Rat
Stabilizer :
0.05% BSA
Storage :
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Subclass :
IgG1 kappa
Synonyms :
Toll-like receptor 9
Target Species :
Human
Background :
Anti-TLR9 CD289 Antibody detects human TLR9 CD289. The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules. The TLR family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition. Ten human homologs of TLRs (TLR1-10) have been described. By using a BLAST search, a cDNA coding for TLR9 has been identified and subsequently isolated. Gene knockout experiments suggest that TLR9 acts as a receptor for unmethylated CpG dinucleotides in the bacterial DNA. Human and mouse TLR9 share an overall amino-acid identity of 75.5%. TLR9 is highly expressed in spleen. Anti-TLR9 CD289 Antibody is ideal for investigators involved in cytokines and growth factor research.
Immunogen Type :
Peptide
Low Endotoxin :
No
Sample Size :
No
Target Domain of Reactivity :
Internal
Application Text :
Flow Cytometry,Immunohistochemistry,Western Blot,
Category :
Primary Antibodies
Conjugation Name :
Unconjugated
UniProt :
Q9NR96
NCBI :
NP_059138.1
Primary Image Name :
TLR9 Flow Cytometry
company information
Rockland Immunochemicals
321 Jones Blvd
Pottstown, PA 19464
tech@rockland.com
https://www.rockland.com/
484-791-3823
headquarters: USA
Rockland Immunochemicals, Inc. produces Phosho-Site Specific Antibodies and Antibody based tools for basic, applied and clinical research. Our laboratory is located west of Philadelphia, Pennsylvania, USA. The technology base of the organization is the experience of its staff scientists in the production and purification of a wide range of antibodies through monoclonal & polyclonal antibody techniques utilizing in vitro and in vivo methods. Rockland's antibodies are suited for individuals performing Western Blotting, ELISA, Immunohistochemistry, Fluorescent Microscopy, High Content Screening and diagnostic kit production. Primary antibodies include Akt Pathway, Apolipoproteins, Apoptosis/Cell Cycle, Cytokines, Cell Signaling, Enzymes, Extracellular Matrix, Transcription Factors (NFkB) and Ubiquitin.