TLR5 Antibody | Rockland Immunochemicals 200-301-I27 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

TLR5 Antibody

catalog :

200-301-I27

quantity :

100 µg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

85B152.5

reactivity :

human, mouse

application :

western blot, flow cytometry

more info or order :

Rockland Immunochemicals product webpage

product information

Catalog Number :

200-301-I27

Name :

Anti-TLR5 (MOUSE) Monoclonal Antibody - 200-301-I27

Display Name :

TLR5 Antibody

Application Note :

Anti-TLR5 Antibody is suitable for use in Flow (I), IHC (p) and WB. Expect a band approximately 97kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.

Buffer :

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Monoclonal

Clone ID :

85B152.5

Concentration Value :

0.5 mg/mL

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

(None)

Size :

100 µg

Default Unit :

µg

Flow Cytometry Dilution :

1-3 µg/10^6 cells

Western Blot Dilution :

1-3 µg/ml

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG2a

Gene Name :

TLR5

Host Animal :

Mouse

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

TLR5 Antibody was produced in mice prepared by repeated immunizations with a synthetic KLH-conjugated peptide corresponding to human TLR5.

Packing Type :

Dry Ice

Physical State :

Liquid

Preservative :

0.05% (w/v) Sodium Azide

Purity and Specificity :

Anti-TLR5 Antibody was purified by Protein G chromatography. A BLAST analysis was used to suggest cross-reactivity with Anti-TLR5 from dog, human and mouse based on 100% homology with the immunizing sequence. Cross-reactivity with Anti-TLR5 from other sources has not been determined.

Species Reactivity :

Human, mouse

Stabilizer :

0.05% BSA

Storage :

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Subclass :

IgG2a kappa

Synonyms :

TIL3, Toll-like receptor 5, Toll/interleukin-1 receptor-like protein 3

Target Species :

Human

Background :

Anti-TLR5 Antibody detects human TLR5. The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules and could constitute an important and unrecognized component of innate immunity in humans. The TLR family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition. TLRs characterized so far activate the MyD88/interleukin-1 receptor-associated kinase (IRAK) signaling pathway. Thirteen homologs of TLRs (TLR1-13) have been described. Toll-like receptor 5 (TLR5) expression is upregulated following exposure to bacteria or to the TLR5 agonist, flagellin. Gram-negative bacteria, stimulate monocyte/macrophage cells in a TLR5-specific, CD14-independent manner. The TLR5 receptor thus appears to be the principal means by which the innate immune system recognizes flagellated bacterial pathogens. Anti-TLR5 Antibody is ideal for investigators involved in cytokines and growth factor research.

Immunogen Type :

Peptide

Low Endotoxin :

Sample Size :

Target Domain of Reactivity :

Internal

Application Text :

Flow Cytometry,Western Blot,

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

UniProt :

O60602

NCBI :

NP_003259.2

Primary Image Name :

TLR5 Flow Cytometry

more info or order :

Rockland Immunochemicals product webpage