TLR2 Antibody | Rockland Immunochemicals 200-301-I23 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

TLR2 Antibody

catalog :

200-301-I23

quantity :

100 µg

price :

349.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

1030A5.138

reactivity :

human

application :

western blot, immunohistochemistry, flow cytometry

product information

Catalog Number :

200-301-I23

Name :

Anti-TLR2 (MOUSE) Monoclonal Antibody - 200-301-I23

Display Name :

TLR2 Antibody

Application Note :

Anti-TLR2 Antibodyis suitbale for use in Flow (CS), Flow (I), IHC (p) and WB. Expect a band approximately 100kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.

Buffer :

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Monoclonal

Clone ID :

1030A5.138

Concentration Value :

0.5 mg/mL

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

(None)

Size :

100 µg

Default Unit :

µg

Flow Cytometry Dilution :

2.5-5 µl/10^6 cells

Immunohistochemistry Dilution :

20 µg/mL (praffin)

Western Blot Dilution :

1:250-1:1000

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG1

Gene Name :

TLR2

Host Animal :

Mouse

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

TLR2 Antibody was produced in mice prepared by repeated immunizations with the extracellular domain of human TLR2 protein.

Packing Type :

Dry Ice

Physical State :

Liquid

Preservative :

0.05% (w/v) Sodium Azide

Purity and Specificity :

Anti-TLR2 Antibody was purified by Protein G chromatography. A BLAST analysis was used to suggest cross-reactivity with Anti-TLR2 from human based on 100% homology with the immunizing sequence. Cross-reactivity withAnti-TLR2 from other sources has not been determined.

Species Reactivity :

Human

Stabilizer :

0.05% BSA

Storage :

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Subclass :

IgG1

Synonyms :

TIL4, Toll-like receptor 2, Toll/interleukin-1 receptor-like protein 4, CD282

Target Species :

Human

Background :

Anti-TLR2 CD282 Antibody detects human TLR2. The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules. The TLR family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition. Ten human homologs of TLRs (TLR1-10) have been described. TLR2 is differentially expressed in human cells. TLR2 is expressed in tonsils, lymph nodes, and appendices, activated B-cells in germinal centers. CD14+ monocytes expressed the highest level of TLR2 followed by CD15+ granulocytes, and CD19+ B-cells, CD3+ T-cells, and CD56+ NK cells did not express TLR2. The expression of TLR2 on different cell types are regulated by different immune response modifiers. For example, LPS, GM-CSF, IL-1, and IL-10 up regulates TLR2 whereas IL-4, IFN-gamma, and TNF down regulate TLR2 expression in monocytes. Anti-TLR2 Antibody is ideal for investigators involved in cytokines and growth factor research.

General Reference :

1. Chuang TH and Ulevitch RJ. Biochim. Biophys. Acta 1518 (1-2): 157-161 (2001). 2. Flo TH, Halaas O, Torp S, Ryan L, Lien E, Dybdahl B, Sundan A, Espevik T. J Leukoc Biol 69(3): 474-481 . 3. Muzio M, Natoli G, Saccani S, Levrero M, and Mantovani A. J. Exp. Med. 187: 2097-2101 (1998). 4. Medzhitov R and Janeway CA. Cell 91: 295-298 (1997). 5. Chuang TH and Ulevitch RJ. Eur Cytokine Netw 11(3): 372-378 (2000). 6. Elzinandes L Azeredo, Patrícia C Neves-Souza, Allan R Alvarenga, Sônia R N I Reis, Amanda Torrentes-Carvalho, Sonia-Maris O Zagne, Rita M R Nogueira, Luzia M Oliveira-Pinto, Claire F Kubelka. Differential regulation of toll-like receptor-2, toll-like receptor-4, CD16 and human leucocyte antigen-DR on peripheral blood monocytes during mild and severe dengue fever. Immunology. 2010 June; 130(2): 202–216.

Immunogen Type :

Peptide

Low Endotoxin :

Sample Size :

Target Domain of Reactivity :

Extracellular

Created On :

1/11/2013 9:56 AM

Modified On :

1/2/2017 9:57 AM

Application Text :

Flow Cytometry,Immunohistochemistry,Western Blot,

Approved for General Distributors :

12/1/2014

Discontinued Item :

Division :

OEM

Launch Date :

1/11/2013

Vendor :

Novus Biologicals

Status :

Active

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

2018 Price :

349.00 USD

UniProt :

O60603

NCBI :

NP_003255.2

Primary Image Name :

TLR2 Flow Cytometry