NOXA Antibody | Rockland Immunochemicals 200-301-H98 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

NOXA Antibody

catalog :

200-301-H98

quantity :

100 µg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

114C307.1

reactivity :

human

application :

western blot, immunohistochemistry

more info or order :

Rockland Immunochemicals product webpage

citations: 2

Reference
Haschka M, Karbon G, Soratroi C, O Neill K, Luo X, Villunger A. MARCH5-dependent degradation of MCL1/NOXA complexes defines susceptibility to antimitotic drug treatment. Cell Death Differ. 2020;: pubmed publisher
Ichikawa N, Alves M, Pfeiffer S, Langa E, HernÃ¡ndez Santana Y, Suzuki H, et al. Deletion of the BH3-only protein Noxa alters electrographic seizures but does not protect against hippocampal damage after status epilepticus in mice. Cell Death Dis. 2017;8:e2556 pubmed publisher

product information

Catalog Number :

200-301-H98

Name :

Anti-NOXA (MOUSE) Monoclonal Antibody - 200-301-H98

Display Name :

NOXA Antibody

Application Note :

Anti-Noxa antibody is suitable for use by IF/ICC, IHC (p) and WB. Expect a band approximately 6 kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.

Buffer :

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Monoclonal

Clone ID :

114C307.1

Concentration Value :

1.0 mg/mL

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

(None)

Size :

100 µg

Default Unit :

µg

Immunohistochemistry Dilution :

5 µg/mL

Western Blot Dilution :

1-2 µg/mL

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG1

Gene Name :

PMAIP1

Host Animal :

Mouse

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

Noxa Antibody was produced in mice prepared by repeated immunizations with the human protein Noxa.

Packing Type :

Dry Ice

Physical State :

Liquid

Preservative :

0.05% (w/v) Sodium Azide

Purity and Specificity :

Anti-Noxa Antibody was purified by Protein G chromatography. A BLAST analysis was used to suggest cross-reactivity with Anti-Noxa from human and mouse based on 100% homology with the immunizing sequence. Cross-reactivity with Anti-Noxa from other sources has not been determined.

Species Reactivity :

Human, Mouse

Storage :

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Subclass :

IgG1 kappa

Synonyms :

NOXA, Phorbol-12-myristate-13-acetate-induced protein 1, PMA-induced protein 1, Immediate-early-response protein APR, Protein Noxa

Target Species :

Human

Background :

Anti-Noxa antibody detects human Noxa. Recently, a BH-3 only member of the Bcl-2 family have been identified in human and mouse and designated as Noxa (for damage). The expression of the Noxa gene involves direct activation of its promoter by p53. Increased expression of Noxa protein occurs in normal thymocytes but not in p53-deficient thymocytes. Noxa cDNA codes for a 103-amino acid protein. The coimmunoprecipitation data suggest that Noxa protein may interact with proteins belonging to the Bcl-2 family, such as, Bcl-XL and Mcl-1. Oda et al. have also shown that blocking the endogenous Noxa induction results in the suppression of apoptosis. Treatment of cells with Noxa antisense oligonucleotides blocks radiation-induced apoptosis suggesting that Noxa may represent a mediator of p53-dependent apoptosis. Anti-Noxa antibody is ideal for investigators involved in apaoptosis research.

Immunogen Type :

Recombinant Protein

Low Endotoxin :

Sample Size :

Application Text :

Immunohistochemistry,Western Blot,

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

UniProt :

Q13794

NCBI :

NP_066950.1

Primary Image Name :

Noxa Immunohistochemistry

more info or order :

Rockland Immunochemicals product webpage