MyoD Antibody | Rockland Immunochemicals 200-301-H94 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

MyoD Antibody

catalog :

200-301-H94

quantity :

100 µg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

5.8A

reactivity :

human, mouse

application :

western blot, immunohistochemistry, immunoprecipitation

more info or order :

Rockland Immunochemicals product webpage

product information

Catalog Number :

200-301-H94

Name :

Anti-MyoD (MOUSE) Monoclonal Antibody - 200-301-H94

Display Name :

MyoD Antibody

Application Note :

Anti-MyoD Antibody is suitable for use in IF/ICC, IHC (frozen), IP and WB. Expect a band approximately 45kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.

Buffer :

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Monoclonal

Clone ID :

5.8A

Concentration Value :

1.0 mg/mL

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

(None)

Size :

100 µg

Default Unit :

µg

Immunohistochemistry Dilution :

5 µg/mL

Immunoprecipitation Dilution :

1 µg/mL

Western Blot Dilution :

1 µg/mL

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG1

Gene Name :

Myod1

Host Animal :

Mouse

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

MyoD Antibody was produced in mice prepared by repeated immunizations with recombinant mouse MyoD protein at an internal amino acid sequence.

Packing Type :

Dry Ice

Physical State :

Liquid

Preservative :

0.02% (w/v) Sodium Azide

Purity and Specificity :

Anti-MyoD Antibody was purified by Protein G chromatography. A BLAST analysis was used to suggest cross-reactivity with Anti-MyoD from Human, Cat, Rat, and Mouse based on 100% homology with the immunizing sequence. Cross-reactivity with Anti-MyoD from other sources has not been determined.

Species Reactivity :

human

Storage :

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Subclass :

IgG1 kappa

Synonyms :

Myod, Myoblast determination protein 1, Class C basic helix-loop-helix protein 1, bHLHc1, Myogenic factor 3, Myf-3

Target Species :

Mouse

Background :

Anti-MyoD Antibody detects MyoD. The Myogenic determination gene (MyoD) was first identified by the virtue of its ability to convert embryonic mouse fibroblast cells to muscle cells. It was subsequently shown that forced expression of MyoD (human homolog is myf 3) gene in a wide variety of normal and neoplastic cells could either convert the cells to muscle cells or activate a set of the otherwise transcriptionally inactive muscle-specific genes in these cells. The regulatory domain of the MyoD gene product lies within a 70 amino acid region and comprises a basic DNA binding motif and a helix-loop-helix (HLH) dimerization motif. Subsequent studies identified three other genes whose products shared sequence homology for the basic HLH domain of MyoD. These are: myf5, myogenin (human homolog is myf4) and myf6 (also known as MRF4 and herculin). Anti-MyoD Antibody is ideal for investigators involved in cell cycle protein, cytokines and growth factors.

Immunogen Type :

Recombinant Protein

Low Endotoxin :

Sample Size :

Target Domain of Reactivity :

Internal

Application Text :

Immunohistochemistry,Immunoprecipitation,Western Blot,

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

UniProt :

P10085

NCBI :

NP_034996.2

Primary Image Name :

MyoD Immunohistochemistry

more info or order :

Rockland Immunochemicals product webpage