MBD3 Antibody | Rockland Immunochemicals 200-301-H92 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

MBD3 Antibody

catalog :

200-301-H92

quantity :

100 µg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

106B691

reactivity :

human

application :

western blot

more info or order :

Rockland Immunochemicals product webpage

product information

Catalog Number :

200-301-H92

Name :

Anti-MBD3 (MOUSE) Monoclonal Antibody - 200-301-H92

Display Name :

MBD3 Antibody

Application Note :

Anti-MBD3 Antibody is suitable for use by WB. Expect a band approximately 33 kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.

Buffer :

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Monoclonal

Clone ID :

106B691

Concentration Value :

1.0 mg/mL

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

(None)

Size :

100 µg

Default Unit :

µg

Western Blot Dilution :

2 µg/mL

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG1

Gene Name :

MBD3

Host Animal :

Mouse

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

MBD3 Antibody was produced in mice prepared by repeated immunizations with human MBD3 along the internal region of the protein.

Packing Type :

Dry Ice

Physical State :

Liquid

Preservative :

0.05% (w/v) Sodium Azide

Purity and Specificity :

Anti-MBD3 Antibody was purified by Protein G chromatography. A BLAST analysis was used to suggest cross-reactivity with Anti-MBD3 from human based on 100% homology with the immunizing sequence. Cross-reactivity with Anti-MBD3 from other sources has not been determined.

Species Reactivity :

Human

Stabilizer :

0.05% BSA

Storage :

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Subclass :

IgG1 kappa

Synonyms :

Methyl-CpG-binding domain protein 3, Methyl-CpG-binding protein MBD3

Target Species :

Human

Background :

Anti-MBD3 Antibody detects human MBD3. DNA methylation, or the addition of methyl groups to cytosine bases in the dinucleotide CpG, is imperative to proper development and regulates gene expression. The methylation pattern involves the enzymatic processes of methylation and demethylation. The demethylation enzyme was recently found to be a mammalian protein, which exhibits demethylase activity associated to a methyl-CpG-binding domain (MBD). The enzyme is able to revert methylated cytosine bases to cytosines within the particular dinucleotide sequence mdCpdG by catalyzing the cleaving of the methyl group as methanol. MeCP2 and MBD1 (PCM1) are first found to repress transcription by binding specifically to methylated DNA. MBD2 and MBD4 (also known as MED1) were later found to colocalize with foci of heavily methylated satellite DNA and believed to mediate the biological functions of the methylation signal. Surprisingly, MBD3 does not bind methylated DNA both in vivo and in vitro. MBD1, MBD2, MBD3, and MBD4 are found to be expressed in somatic tissues, but the expression of MBD1 and MBD2 is reduced or absent in embryonic stem cells, which are known to be deficient in MeCP1 activity. MBD4 have homology to bacterial base excision repair DNA N-glycosylases/lyases. In some microsatellite unstable tumors MBD4 is mutated at an exonic polynucleotide tract. Anti-MBD3 Antibody is ideal for investigators involved in Epigenetic, DNA damage and repair, and cell cycle protein research.

Immunogen Type :

Peptide

Low Endotoxin :

Sample Size :

Target Domain of Reactivity :

Internal

Application Text :

Western Blot,

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

UniProt :

O95983

NCBI :

NP_003917.1

Primary Image Name :

MBD3 Western Blot

more info or order :

Rockland Immunochemicals product webpage