IKK gamma Antibody | Rockland Immunochemicals 200-301-H86 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

IKK gamma Antibody

catalog :

200-301-H86

quantity :

100 µg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

46B844

reactivity :

human

application :

western blot, flow cytometry

more info or order :

Rockland Immunochemicals product webpage

product information

Catalog Number :

200-301-H86

Name :

Anti-IKKɣ (MOUSE) Monoclonal Antibody - 200-301-H86

Display Name :

IKK gamma Antibody

Application Note :

Anti-IKKgamma antibody is suitable for use in Flow (I) and WB. Expect a band approximately 48kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.

Buffer :

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Monoclonal

Clone ID :

46B844

Concentration Value :

0.5 mg/mL

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

(None)

Size :

100 µg

Default Unit :

µg

Flow Cytometry Dilution :

0.1-0.5/10^6 cells

Western Blot Dilution :

2 µg/mL

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG1

Gene Name :

IKBKG

Host Animal :

Mouse

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

IKK gamma Antibody was produced in mice prepared by repeated immunizations with human IKKgamma (NEMO) protein at full-length.

Packing Type :

Dry Ice

Physical State :

Liquid

Preservative :

0.05% (w/v) Sodium Azide

Purity and Specificity :

Anti-IKKg Antibody was purified by Protein G chromatography. A BLAST analysis was used to suggest cross-reactivity with IKKg from Human based on 100% homology with the immunizing sequence. Cross-reactivity with Anti-IKKg from other sources has not been determined.

Species Reactivity :

Human

Stabilizer :

0.05% BSA

Storage :

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Subclass :

IgG1 lambda

Synonyms :

FIP3, NEMO, NF-kappa-B essential modulator, NEMO, FIP-3, IkB kinase-associated protein 1, IKKAP1, Inhibitor of nuclear factor kappa-B kinase subunit gamma, I-kappa-B kinase subunit gamma, IKK-gamma, IKKG, IkB kinase subunit gamma, NF-kappa-B essential modifier

Target Species :

Human

Background :

Anti-IKK gamma detects human IKKgamma. NF-kappaB (nuclear factor kB) is sequestered in the cytoplasm by IkB family of inhibitory proteins that mask the nuclear localization signal of NF-kappaB thereby preventing translocation of NF-kB to the nucleus. External stimuli such as tumor necrosis factor or other cytokines results in phosphorylation and degradation of IkB releasing NF-kB dimers. NF-kB dimer subsequently translocates to the nucleus and activates target genes. Synthesis of IkBa is autoregulated. IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, IKKalpha, IKKbeta, and IKKgamma (NEMO). NEMO (NF-kB Essential MOdulator) preferentially interacts with IKK2/b and is required for activation of IKK complex. Recent data suggest that the human T-cell leukemia virus type I Tax oncoprotein that activates NF-kB binds neither to IKKalpha nor IKKbeta, but complexes directly with NEMO. This suggests that NEMO may be a key molecule acting as an adapter for onco-protein specific signaling to IKKa and IKKb. Anti-IKKgamma antibody is ideal for investigators involved in NFkappaB, kinase and growth factor research.

Immunogen Type :

Native Protein

Low Endotoxin :

Sample Size :

Application Text :

Flow Cytometry,Western Blot,

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

UniProt :

Q9Y6K9

NCBI :

NP_001093326.2

Primary Image Name :

IKKgamma Flow Cytometry

more info or order :

Rockland Immunochemicals product webpage