IKK epsilon Antibody | Rockland Immunochemicals 200-301-H85 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

IKK epsilon Antibody

catalog :

200-301-H85

quantity :

100 ug

price :

329.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

72B587

reactivity :

human

application :

western blot, flow cytometry

product information

Catalog Number :

200-301-H85

Name :

Anti-IKK_ (MOUSE) Monoclonal Antibody - 200-301-H85

Display Name :

IKK epsilon Antibody

Application Note :

Anti-IKK_ antibody is suitbale for use in Flow (I) and WB. Expect a band approximately 80kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.

Buffer :

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Monoclonal

Clone ID :

72B587

Concentration Value :

0.5 mg/mL

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

(None)

Size :

100 ug

Default Unit :

Flow Cytometry Dilution :

0.1-0.5 xB5g/mL

Western Blot Dilution :

1-3 xB5g/mL

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG2a

Gene Name :

IKBKB

Host Animal :

Mouse

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

IKK epsilon Antibody was produced in mice prepared by repeated immunizations with a synthetic peptide corresponding to internal amino acids with human IKK epsilon protein.

Packing Type :

Dry Ice

Physical State :

Liquid

Preservative :

0.05% (w/v) Sodium Azide

Purity and Specificity :

Anti-IKK_ Antibody was purified by Protein G chromatography. A BLAST analysis was used to suggest cross-reactivity with IKKe from Human, Mouse, and rat based on 100% homology with the immunizing sequence. Cross-reactivity with Anti-IKKe from other sources has not been determined.

Species Reactivity :

Human

Stabilizer :

0.05% BSA

Storage :

Store vial at -20 xA1 C prior to opening. Aliquot contents and freeze at -20 xA1 C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 xA1 C as an undiluted liquid. Dilute only prior to immediate use.

Subclass :

IgG2a kappa

Synonyms :

Inhibitor of nuclear factor kappa-B kinase subunit epsilon, I-kappa-B kinase epsilon, IKK-E, IKK-epsilon, IkBKE, 2.7.11.10, Inducible I kappa-B kinase, IKK-i

Target Species :

Human

Background :

Anti-IKK_ antibody detects human IKKe IKKi TBK1. NF-kB (nuclear factor kB) is sequestered in the cytoplasm by IkB family of inhibitory proteins that mask the nuclear localization signal of NF-kB thereby preventing translocation of NF-kB to the nucleus. External stimuli such as tumor necrosis factor or other cytokines results in phosphorylation and degradation of IkB releasing NF-kB dimers. NF-kB dimer subsequently translocates to the nucleus and activates target genes. Synthesis of IkBa is autoregulated. IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, IKK1/a, IKK2/b, and IKK3/g. Using a subtractive hybridization technique, a novel kinase, IKK epsilon has been isolated. LPS increases IKK epsilon mRNA level in mouse macrophage cell lines. The IKK epsilon protein has significant sequence homology with kinase domains of IKK1/a and IKK2/b. Overexpression of wild type IKK epsilon in cells phosphorylates Ser32 and Ser36 of IkBa. Anti-IKKe IKKi TBK1 antibody is ideal for investigators involved kinase and growth factor research.

General Reference :

1. Mercurio, F., et al. Science 278: 860 (1997). 2. Shimada T, et al. Int. Immunol 11(8):1357-1362 (1999). 3. Verma, I.M., Stevenson, J.K., Schwarz, EM., Van Antwerp, D. & Miyamoto, S. Genes. Dev. 9: 2723-2735 (1995). 4. Verma, I. And Stevenson, J.K. Proc. Nat. Acad. Sci. USA 94: 11758 (1997). 5. Didonato, J.A., Hayakawa, M., Rothwarf, D.M., Zandi, E., and Karin, M. Nature 388: 548- (1997). 6. Regnier, C.H., Ho, Y.S., Gao, X., Goeddel, D.V., Cao, Z., and Rothe, M. Cell 90, 373 (1997).

Immunogen Type :

Peptide

Low Endotoxin :

Sample Size :

Target Domain of Reactivity :

Internal

Created On :

1/11/2013 9:56 AM

Modified On :

6/29/2015 9:58 AM

Application Text :

Flow Cytometry,Western Blot

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

2015 List Price :

319.00

2016 List Price :

329.00 USD

Uniprot :

Q14164

NCBI :

NP_001180250.1

Primary Image Name :

IKK epsilon IKKi TBK1 Flow Cytometry

Primary Image Legend :

Flow Cytometry of Mouse Anti-IKK epsilon IKKi TBK1 antibody. Cells: 10^6 NIH 3T3 cells. Stimulation: none. Primary Antibody: IKK epsilon antibody at 0.1 xB5g/mL (red) and isotype control (green). Secondary Antibody: goat anti-mouse IgG FITC.