IKK alpha Antibody | Rockland Immunochemicals 200-301-H82 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

IKK alpha Antibody

catalog :

200-301-H82

quantity :

100 µg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

14A231

reactivity :

human

application :

western blot, immunohistochemistry, immunoprecipitation, flow cytometry

more info or order :

Rockland Immunochemicals product webpage

product information

Catalog Number :

200-301-H82

Name :

Anti-IKKα (MOUSE) Monoclonal Antibody - 200-301-H82

Display Name :

IKK alpha Antibody

Application Note :

Anti-IKK alpha antibody is suitable for use in Flow (I), IHC (p), IP and WB. Expect a band approximately 85-90kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.

Buffer :

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Monoclonal

Clone ID :

14A231

Concentration Value :

1.0 mg/mL

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

(None)

Size :

100 µg

Default Unit :

µg

Flow Cytometry Dilution :

0.25-0.5 µg/10^6 cells

Immunohistochemistry Dilution :

5 µg/mL

Immunoprecipitation Dilution :

1-2 µg/mL

Western Blot Dilution :

1 µg/mL

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG1

Gene Name :

CHUK

Host Animal :

Mouse

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

IKK alpha Antibody was produced in mice prepared by repeated immunizations by a His-tagged human IKK alpha protein at full-length.

Packing Type :

Dry Ice

Physical State :

Liquid

Preservative :

0.05% (w/v) Sodium Azide

Purity and Specificity :

Anti-IKKα Antibody was purified by Protein G chromatography. A BLAST analysis was used to suggest cross-reactivity with IKKα from Human, Mouse and New World Monkey based on 100% homology with the immunizing sequence. Cross-reactivity with Anti-IKKα from other sources has not been determined.

Species Reactivity :

Human

Storage :

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Subclass :

IgG1 kappa

Synonyms :

IKKA, TCF16, Inhibitor of nuclear factor kappa-B kinase subunit alpha, I-kappa-B kinase alpha, IKK-A, IKK-alpha, IkBKA, IkappaB kinase, 2.7.11.10, Conserved helix-loop-helix ubiquitous kinase, I-kappa-B kinase 1, IKK1, Nuclear factor NF-kappa-B inhibitor kinase alpha, NFKBIKA, Transcription factor 16, TCF-16

Target Species :

Human

Background :

Anti-IKK alpha antibody detects human IKK alpha. NF-kB (nuclear factor kB) is sequestered in the cytoplasm by IkB family of inhibitory proteins that mask the nuclear localization signal of NF-kB thereby preventing translocation of NF-kB to the nucleus. External stimuli such as tumor necrosis factor or other cytokines results in phosphorylation and degradation of IkB releasing NF-kB dimers. NF-kB dimer subsequently translocates to the nucleus and activates target genes. Synthesis of IkBa is autoregulated. IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, IKK alpha, IKK beta, and IKK gamma. In vitro, IKK alpha and IKK beta can form homo- and heterodimers that can phosphorylate IkBs at the regulatory serine residues directly. IKK alpha and IKK beta are phosphorylated by NF-kB-inducing kinase (NIK) and MAP kinase kinase kinase-1 (MEKK1), respectively. Targeted disruption of IKK alpha gene in mice results in skin and limb abnormalities and death of newborns. Anti-IKK alpha antibody is ideal for investigators involved in cytokines and growth factor and NFkappaB research.

Immunogen Type :

Recombinant Protein

Low Endotoxin :

Sample Size :

Application Text :

Flow Cytometry,Immunohistochemistry,Immunoprecipitation,Western Blot,

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

UniProt :

O15111

NCBI :

NP_001269.3

Primary Image Name :

IKK alpha Immunohistochemistry

more info or order :

Rockland Immunochemicals product webpage