Dnmt3a Antibody | Rockland Immunochemicals 200-301-H70 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

Dnmt3a Antibody

catalog :

200-301-H70

quantity :

100 µg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

64B1446

reactivity :

human, mouse

application :

western blot

more info or order :

Rockland Immunochemicals product webpage

product information

Catalog Number :

200-301-H70

Name :

Anti-Dnmt3a (MOUSE) Monoclonal Antibody - 200-301-H70

Display Name :

Dnmt3a Antibody

Application Note :

Anti-Dnmt3a Antibody is suitable for use in ChIP, IF/ICC, IHC (p) and WB. Expect a band approximately 120 kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.

Buffer :

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Monoclonal

Clone ID :

64B1446

Concentration Value :

0.5 mg/mL

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

(None)

Size :

100 µg

Default Unit :

µg

IF Microscopy Dilution :

5 µg/mL

Western Blot Dilution :

2 µg/mL

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG1

Gene Name :

Dnmt3a

Host Animal :

Mouse

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

Dnmt3a Antibody was produced in mice prepared by repeated immunizations with bacteria expressed near the C-terminus recombinant mouse Dnmt3a protein.

Packing Type :

Dry Ice

Physical State :

Liquid

Preservative :

0.05% (w/v) Sodium Azide

Purity and Specificity :

Anti-Dnmt3a Antibody was purified by Protein G chromatography. A BLAST analysis was used to suggest cross-reactivity with Dnmt3a from human and mouse based on 100% homology with the immunizing sequence. Cross-reactivity with Anti-Dnmt3a from other sources has not been determined.

Species Reactivity :

Human

Stabilizer :

0.05% BSA

Storage :

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Subclass :

IgG1 kappa

Synonyms :

DNA (cytosine-5)-methyltransferase 3A, Dnmt3a, 2.1.1.37, DNA methyltransferase HsaIIIA, DNA MTase HsaIIIA, M.HsaIIIA

Target Species :

Mouse

Background :

Anti-Dnmt3a Antibody detects mouse Dnmt3a. Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, 3 families of mammalian DNA methyltransferase genes have been identified which include Dnmt1, Dnmt2 and Dnmt3. Dnmt1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. Dnmt2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. The Dnmt3 family members, Dnmt3a and Dnmt3b, are strongly expressed in ES cells but their expression is down regulated in differentiating ES cells and is low in adult somatic tissue. Recently, it has been shown that naturally occurring mutations of Dnmt3b gene occurs in patients with a rare autosomal recessive disorder, termed ICF (immunodeficiency, centromeric instability, and facial anomalies) syndrome. Anti-Dnmt3a Antibody is ideal for investigators involved in caner, epigentics, and cell cycle protein research.

Immunogen Type :

Recombinant Protein

Low Endotoxin :

Sample Size :

Target Domain of Reactivity :

C-Terminus

Application Text :

IF Microscopy,Western Blot,

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

UniProt :

O88508

NCBI :

NP_031898.1

Primary Image Name :

Dnmt3a Immunofluorescence

more info or order :

Rockland Immunochemicals product webpage