DNMT1 Antibody | Rockland Immunochemicals 200-301-H69 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

DNMT1 Antibody

catalog :

200-301-H69

quantity :

100 µg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

60B1220.1

reactivity :

human, mouse

application :

western blot, immunohistochemistry

more info or order :

Rockland Immunochemicals product webpage

product information

Catalog Number :

200-301-H69

Name :

Anti-Dnmt1 (MOUSE) Monoclonal Antibody - 200-301-H69

Display Name :

DNMT1 Antibody

Application Note :

Anti-DNMT1 antibody is suitable for use in ChIP, IHC (p), IP and WB. Expect a band approximately 183 kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.

Buffer :

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Monoclonal

Clone ID :

60B1220.1

Concentration Value :

1.0 mg/mL

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

(None)

Size :

100 µg

Default Unit :

µg

Immunohistochemistry Dilution :

1-2 µg/mL

Western Blot Dilution :

0.1-0.5 µg/mL

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG1

Gene Name :

DNMT1

Host Animal :

Mouse

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

Dnmt1 Antibody was produced in mice prepared by repeated immunizations with a synthetic peptide corresponding to internal amino acids with Dnmt1 human protein.

Packing Type :

Dry Ice

Physical State :

Liquid

Preservative :

0.05% (w/v) Sodium Azide

Purity and Specificity :

Anti-Dnmt1 Antibody was purified by Protein G chromatography. A BLAST analysis was used to suggest cross-reactivity with Dnmt1 from human, mouse and Zebrafish based on 100% homology with the immunizing sequence. Cross-reactivity with Anti-Dnmt1 from other sources has not been determined.

Species Reactivity :

Human, Mouse

Stabilizer :

0.05% BSA

Storage :

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Subclass :

IgG1 kappa

Synonyms :

AIM, CXXC9, DNMT, DNA (cytosine-5)-methyltransferase 1, CXXC-type zinc finger protein 9, DNA methyltransferase HsaI

Target Species :

Human

Background :

Anti-Dnmt1 antibody detects human Dnmt1. Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, 3 families of mammalian DNA methyltransferase genes have been identified which include Dnmt1, Dnmt2 and Dnmt3. Dnmt1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. Dnmt2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. The Dnmt3 family members, Dnmt3a and Dnmt3b, are strongly expressed in ES cells but their expression is down regulated in differentiating ES cells and is low in adult somatic tissue. Dnmt1 co-purifies with the retinoblastoma (Rb) tumor suppressor gene product, E2F1, and HDAC1. Dnmt1 also cooperates with Rb to repress transcription from promoters containing E2F-binding sites suggesting a link between DNA methylation, histone deacetylase and sequence-specific DNA binding activity, as well as a growth-regulatory pathway that is disrupted in nearly all cancer cells. Anti-Dnmt1 antibody is ideal for investigators involved in cancer, cell cycle protein research and epigenetics.

Immunogen Type :

Peptide

Low Endotoxin :

Sample Size :

Target Domain of Reactivity :

Internal

Application Text :

Immunohistochemistry,Western Blot,

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

UniProt :

P26358

NCBI :

NP_001124295.1

Primary Image Name :

Dnmt1 Immunohistochemistry

more info or order :

Rockland Immunochemicals product webpage