Caspase-8 Antibody | Rockland Immunochemicals 200-301-H64 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

Caspase-8 Antibody

catalog :

200-301-H64

quantity :

100 µg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

90A992

reactivity :

human

application :

western blot, immunohistochemistry, flow cytometry

more info or order :

Rockland Immunochemicals product webpage

product information

Catalog Number :

200-301-H64

Name :

Anti-Caspase-8 (MOUSE) Monoclonal Antibody - 200-301-H64

Display Name :

Caspase-8 Antibody

Application Note :

Anti-Caspase-8 antibody is suitable for use by Flow (I), IHC (p), and WB. Expect a band approximately 55 kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.

Buffer :

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Monoclonal

Clone ID :

90A992

Concentration Value :

1.0 mg/mL

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

(None)

Size :

100 µg

Default Unit :

µg

Flow Cytometry Dilution :

0.1-0.5 µg/mL

Immunohistochemistry Dilution :

5 µg/mL

Western Blot Dilution :

0.5-2 µg/mL

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG1

Gene Name :

Casp8

Host Animal :

Mouse

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

Caspase-8 Antibody was produced in mice prepared by repeated immunizations with amino acids at the internal portion of human Caspase-8.

Packing Type :

Dry Ice

Physical State :

Liquid

Preservative :

0.05% (w/v) Sodium Azide

Purity and Specificity :

Anti-Caspase-8 Antibody was purified by Protein G chromatography. A BLAST analysis was used to suggest cross-reactivity with Anti-Caspase-8 from Chimpanzee, Human, Rhesus Monkey and Mouse based on 100% homology with the immunizing sequence. Cross-reactivity with Anti-Caspase-8 from other sources has not been determined.

Species Reactivity :

Human

Stabilizer :

0.05% BSA

Storage :

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Subclass :

IgG1

Synonyms :

MCH5Caspase-8, CASP-8, CASP8, 3.4.22.61, Apoptotic cysteine protease, Apoptotic protease Mch-5, CAP4, FADD-homologous ICE/ced-3-like protease, FADD-like ICE, FLICE, ICE-like apoptotic protease 5, MORT1-associated ced-3 homolog, MACH, Caspase-8 subunit p18, Caspase-8 subunit p10

Target Species :

Human

Background :

Anti-Caspase-8 detects human Caspase. Caspases are a family of cysteine proteases that are key mediators of programmed cell death or apoptosis. The precursor form of all caspases is composed of a prodomain, and large and small catalytic subunits. The active forms of caspases are generated by several stimuli including ligand-receptor interactions, growth factor deprivation and inhibitors of cellular functions. All known caspases require cleavage adjacent to aspartates to liberate one large and one small subunit, which associate into a2b2 tetramer to form the active enzyme. Caspase-8 (FLICE) forms a direct link between the activation of CD95 and the caspase pathway. Overexpression of Caspase-8 induces apoptosis, which can be blocked by inhibitors specific for the ICE family. Anti-Caspase-8 antibody is ideal for investigators involved in apoptosis research.

Immunogen Type :

Peptide

Low Endotoxin :

Sample Size :

Target Domain of Reactivity :

Internal

Application Text :

Flow Cytometry,Immunohistochemistry,Western Blot,

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

UniProt :

Q14790

NCBI :

NP_001073593.1

Primary Image Name :

Caspase-8 Immunohistochemistry

more info or order :

Rockland Immunochemicals product webpage