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company name :
Rockland Immunochemicals
product type :
antibody
product name :
A20 Antibody
catalog :
200-301-H52
quantity :
100 µg
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
59A426
reactivity :
human
application :
western blot, immunoprecipitation
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product information
Catalog Number :
200-301-H52
Name :
Anti-A20 (MOUSE) Monoclonal Antibody - 200-301-H52
Display Name :
A20 Antibody
Application Note :
Anti-A20 antibody is suitable for use in Flow (I), IF/ICC, IHC (p), IP and WB. Expect a band approximately 90kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.
Buffer :
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Clonality :
Monoclonal
Clone ID :
59A426
Concentration Value :
1.0 mg/mL
Concentration Definition :
by UV absorbance at 280 nm
Conjugation :
(None)
Size :
100 µg
Default Unit :
µg
Immunoprecipitation Dilution :
1-2 µg/mL
Western Blot Dilution :
2-4 µg/mL
Expiration :
Expiration date is one (1) year from date of opening.
Format :
IgG1
Gene Name :
TNFAIP3
Host Animal :
Mouse
General Disclaimer Note :
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Immunogen :
A20 Antibody was produced in mice by repeated immunizations with recombinant human A20 protein at the c-terminus.
Packing Type :
Dry Ice
Physical State :
Liquid
Preservative :
0.05% (w/v) Sodium Azide
Purity and Specificity :
Anti-A20 Antibody was purified by Protein G chromatography. A BLAST analysis was used to suggest cross-reactivity with Anti-A20 from Human, Mouse and rat based on 100% homology with the immunizing sequence. Cross-reactivity with Anti-A20 from other sources has not been determined.
Species Reactivity :
Human
Stabilizer :
0.05% BSA
Storage :
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Subclass :
IgG1 kappa
Synonyms :
OTUD7C, Tumor necrosis factor alpha-induced protein 3, TNF alpha-induced protein 3, OTU domain-containing protein 7C, A20p50, A20p37
Target Species :
Human
Background :
Anti-A20 antibody detects human A20. A20 (TNFAIP3) is an important regulator of proinflammatory signaling pathways, including NK-kB sginaling. A20 is upregulated through NF-kB (p50/p55) activation, and subsequently down regulates NF-kB through its dual function as a deubiquitinase and ubiquitin ligase. The A20 monoclonal antibody has been instrumental in discovering various nuances of NF-kB regulation and key to defining A20 biology. Anti-A20 antibody is ideal for investigators involved in NFkappaB and Signaling research.
Immunogen Type :
Recombinant Protein
Low Endotoxin :
No
Sample Size :
No
Target Domain of Reactivity :
C-Terminus
Application Text :
Immunoprecipitation,Western Blot,
Category :
Primary Antibodies
Conjugation Name :
Unconjugated
UniProt :
P21580
NCBI :
NP_001257436.1
Primary Image Name :
A20 Western Blot
more info or order :
company information

Rockland Immunochemicals
321 Jones Blvd
Pottstown, PA 19464
Pottstown, PA 19464
tech@rockland.com
https://www.rockland.com/484-791-3823
headquarters: USA
Rockland Immunochemicals, Inc. produces Phosho-Site Specific Antibodies and Antibody based tools for basic, applied and clinical research. Our laboratory is located west of Philadelphia, Pennsylvania, USA. The technology base of the organization is the experience of its staff scientists in the production and purification of a wide range of antibodies through monoclonal & polyclonal antibody techniques utilizing in vitro and in vivo methods. Rockland's antibodies are suited for individuals performing Western Blotting, ELISA, Immunohistochemistry, Fluorescent Microscopy, High Content Screening and diagnostic kit production. Primary antibodies include Akt Pathway, Apolipoproteins, Apoptosis/Cell Cycle, Cytokines, Cell Signaling, Enzymes, Extracellular Matrix, Transcription Factors (NFkB) and Ubiquitin.
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