product summary
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company name :
Rockland Immunochemicals
product type :
antibody
product name :
MEK1 phosphoS222/MEK2 phosphoS226 Antibody
catalog :
200-301-GZ6
quantity :
100 µg
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
17C9.E1.H2.D7.H7.D5
reactivity :
human
application :
western blot, ELISA
product information
Catalog Number :
200-301-GZ6
Name :
Anti-MEK1 pS222/MEK2 pS226 Monoclonal Antibody - 200-301-GZ6
Display Name :
MEK1 phosphoS222/MEK2 phosphoS226 Antibody
Application Note :
Anti-MEK1 pS222/MEK2 pS226 (MOUSE) antibody is suitable for use in Western Blotting. Specific conditions of reactivity should be optimized by the end user. Expect a band of approximately 43.5 kDa.
Buffer :
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Clonality :
Monoclonal
Clone ID :
17C9.E1.H2.D7.H7.D5
Concentration Value :
1.0 mg/mL
Concentration Definition :
by UV absorbance at 280 nm
Conjugation :
(None)
Size :
100 µg
Default Unit :
µg
ELISA Dilution :
1:40,000
Western Blot Dilution :
1µg/mL
Expiration :
Expiration date is one (1) year from date of opening.
Format :
IgG
Gene Name :
MAP2K1/MAP2K2
Host Animal :
Mouse
General Disclaimer Note :
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Immunogen :
Anti-MEK1 pS222/MEK2 pS226 Monoclonal Antibody was produced in mice by repeated immunizations with synthetic peptide corresponding to amino acid residues surrounding the S222-226 phosphorylation site conjugated to KLH.
Packing Type :
Dry Ice
Physical State :
Liquid (sterile filtered)
Preservative :
0.01% (w/v) Sodium Azide
Purity and Specificity :
This protein A purified mouse monoclonal antibody specifically binds to the human MEK1 pS222 and MEK2 pS226 phosphorylated sites. Anti-MEK1 pS222/MEK2 pS226 is purified from tissue culture supernatant by protein A purification. Cross reactivity is expected to occur with human, mouse, and rat based on sequence identity of the peptide immunogen.
Species Reactivity :
Human, mouse, rat
Storage :
Store vial at -20° C prior to opening. This product is stable at 4° C as an undiluted liquid. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use.
Subclass :
IgG1 lambda
Synonyms :
mouse anti-MEK1 pS222/MEK2 pS226 Antibody, MAP2K, MEK, MEK1, MKK1, PRKMK1, MEK-1 Antibody, MAP2K2 MEK2, MKK2, PRKMK2, CFC4, MEK-2 Antibody, MEK1 and MEK2 antibody, phosphor MEK1/2
Target Species :
Human
Background :
MEK1 pS222/MEK2 pS226 antibody detects MEK1 and MEK2. Mitogen-activated protein kinase kinase 1, (also known as MKK or MEK1), and Mitogen-activated protein kinase kinase 2, (also known as MEK2 or MKK2), are integral components of the MAP kinase cascade that regulates cell growth and differentiation. This pathway also plays a key role in synaptic plasticity in the brain. Activated MEK 1 and 2 acts as a dual specificity kinase phosphorylating both a threonine and a tyrosine residue on MAP kinase. The MEK1 antibody is ideal for investigators involved in Neuroscience, Cell Signaling and Cancer Research.
Immunogen Type :
Peptide
Low Endotoxin :
No
Post Translational Modification :
Phosphorylation
Sample Size :
No
Target Domain of Reactivity :
Internal
Application Text :
ELISA,Western Blot,
Category :
Primary Antibodies
Conjugation Name :
Unconjugated
UniProt :
Q02750
Primary Image Name :
Western Blot of MEK1 pS222 Antibody
company information
Rockland Immunochemicals
321 Jones Blvd
Pottstown, PA 19464
tech@rockland.com
https://www.rockland.com/
484-791-3823
headquarters: USA
Rockland Immunochemicals, Inc. produces Phosho-Site Specific Antibodies and Antibody based tools for basic, applied and clinical research. Our laboratory is located west of Philadelphia, Pennsylvania, USA. The technology base of the organization is the experience of its staff scientists in the production and purification of a wide range of antibodies through monoclonal & polyclonal antibody techniques utilizing in vitro and in vivo methods. Rockland's antibodies are suited for individuals performing Western Blotting, ELISA, Immunohistochemistry, Fluorescent Microscopy, High Content Screening and diagnostic kit production. Primary antibodies include Akt Pathway, Apolipoproteins, Apoptosis/Cell Cycle, Cytokines, Cell Signaling, Enzymes, Extracellular Matrix, Transcription Factors (NFkB) and Ubiquitin.