product summary
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company name :
Rockland Immunochemicals
product type :
antibody
product name :
Trpm7 Antibody
catalog :
200-301-G57
quantity :
100 µg
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
S74-25
reactivity :
human, mouse
application :
western blot, immunohistochemistry
product information
Catalog Number :
200-301-G57
Name :
Anti-Trpm7 (MOUSE) Monoclonal Antibody - 200-301-G57
Display Name :
Trpm7 Antibody
Application Note :
Anti-TRPM7 Antibody is suitable for use in WB, IHC, and IF/ICC microscopy. Expect a band approximately ~220kDa on specific lysates, in extracts of cells transiently transfected with TrpM7. Specific conditions for reactivity should be optimized by the end user.
Buffer :
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Clonality :
Monoclonal
Clone ID :
S74-25
Concentration Value :
1.0 mg/mL
Concentration Definition :
by UV absorbance at 280 nm
Conjugation :
(None)
Size :
100 µg
Default Unit :
µg
Immunohistochemistry Dilution :
1:1000
IF Microscopy Dilution :
1:100
Western Blot Dilution :
1:1000
Expiration :
Expiration date is one (1) year from date of opening.
Format :
IgG1
Gene Name :
Trpm7
Host Animal :
Mouse
General Disclaimer Note :
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Immunogen :
TRPM7 Antibody was produced in mice by repeated immunizations raised against a fusion proteins corresponding to the c-terminus region of mouse TrpM7.
Packing Type :
Dry Ice
Physical State :
Liquid (sterile filtered)
Preservative :
0.09% (w/v) Sodium Azide
Purity and Specificity :
Anti-TRPM7 Antibody was purified by Protein G chromatography. A BLAST analysis was used to suggest cross-reactivity with TRPM7 from Human, Rat, and Mouse based on 100% homology with the immunizing sequence. No cross-reactivity against TrpM6. Cross-reactivity with TRPM7 from other sources has not been determined. Channels and Transporters research.
Species Reactivity :
Human
Stabilizer :
50% (v/v) Glycerol
Storage :
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Subclass :
IgG1
Synonyms :
CHAK antibody, CHAK1 antibody, LTRPC7 antibody, TRP PLIK antibody, Channel kinase 1 antibody, Transient receptor potential cation channel subfamily M member 7 antibody, Channel-kinase 1 antibody, Long transient receptor potential channel 7 antibody, LTrpC-7 antibody, Transient receptor potential-phospholipase C-interacting kinase antibody, TRP-PLIK antibody, Trpm7 antibody
Target Species :
Mouse
Background :
TRPs, mammalian homologs of the Drosophila transient receptor potential (trp) protein, are ion channels that are thought to mediate capacitative calcium entry into the cell. TRP-PLIK is a protein that is both an ion channel and a kinase. As a channel, it conducts calcium and monovalent cations to depolarize cells and increase intracellular calcium. As a kinase, it is capable of phosphorylating itself and other substrates. The kinase activity is necessary for channel function, as shown by its dependence on intracellular ATP and by the kinase mutants.
Immunogen Type :
Peptide
Low Endotoxin :
No
Sample Size :
No
Target Domain of Reactivity :
C-Terminus
Application Text :
Immunohistochemistry,IF Microscopy,Western Blot,
Category :
Primary Antibodies
Conjugation Name :
Unconjugated
UniProt :
Q923J1
NCBI :
NP_001157797.1
Primary Image Name :
TRPM7 Immunofluorescence
company information
Rockland Immunochemicals
321 Jones Blvd
Pottstown, PA 19464
tech@rockland.com
https://www.rockland.com/
484-791-3823
headquarters: USA
Rockland Immunochemicals, Inc. produces Phosho-Site Specific Antibodies and Antibody based tools for basic, applied and clinical research. Our laboratory is located west of Philadelphia, Pennsylvania, USA. The technology base of the organization is the experience of its staff scientists in the production and purification of a wide range of antibodies through monoclonal & polyclonal antibody techniques utilizing in vitro and in vivo methods. Rockland's antibodies are suited for individuals performing Western Blotting, ELISA, Immunohistochemistry, Fluorescent Microscopy, High Content Screening and diagnostic kit production. Primary antibodies include Akt Pathway, Apolipoproteins, Apoptosis/Cell Cycle, Cytokines, Cell Signaling, Enzymes, Extracellular Matrix, Transcription Factors (NFkB) and Ubiquitin.