Rhodopsin Antibody | Rockland Immunochemicals 200-301-G37 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

Rhodopsin Antibody

catalog :

200-301-G37

quantity :

100 µg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

1D4

reactivity :

human, rat, bovine

application :

western blot, ELISA, immunohistochemistry, immunoprecipitation

more info or order :

Rockland Immunochemicals product webpage

product information

Catalog Number :

200-301-G37

Name :

Anti-Rhodopsin (MOUSE) Monoclonal Antibody - 200-301-G37

Display Name :

Rhodopsin Antibody

Application Note :

Anti-Rhodopsin Antibody is recommended for use in IF microscopy, IHC, IP, WB, and ELISA. Expect a band approximately ~40kDa corresponding to specific lysates. Binds specifically to the N-terminus of Rhodopsin. Specific conditions for reactivity should be optimized by the end user.

Buffer :

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Monoclonal

Clone ID :

1D4

Concentration Value :

1 mg/mL

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

(None)

Size :

100 µg

Default Unit :

µg

ELISA Dilution :

User Optimized

Immunohistochemistry Dilution :

1:100

IF Microscopy Dilution :

User Optimized

Immunoprecipitation Dilution :

User Optimized

Western Blot Dilution :

1:1000

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG1

Gene Name :

RHO

Host Animal :

Mouse

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

Rhodopsin Antibody was produced in mice by repeated immunizations raised against bovine Rhodopsin.

Packing Type :

Dry Ice

Physical State :

Liquid (sterile filtered)

Purity and Specificity :

Anti-Rhodopsin Antibody was purified by Protein G chromatography. A BLAST analysis was used to suggest cross-reactivity with Rhodopsin from all vertebrates based on 100% homology with the immunizing sequence. Cross-reactivity with Rhodopsin from other sources has not been determined. No known reactivity to other proteins. Recognizes Rhodopsin (native and recombinant forms). Binds specifically to the C-terminal epitope -T-E-T-S-Q-V-A-P-A-(COOH).

Species Reactivity :

human, rat

Stabilizer :

50% (v/v) Glycerol

Storage :

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Subclass :

IgG1

Synonyms :

OPN2 Antibody, opsd Antibody, opsin 2 Antibody, opsin 2 rod pigment Antibody, opsin2 Antibody, RHO Antibody, RP4 Antibody, MGC138309 Antibody, Retinitis Pigmentosa 4 Antibody, Rhodopsin Antibody

Target Species :

Bovine

Background :

Rhodopsin consists of the protein moiety opsin and a reversibly covalently bound cofactor, retinal. Opsin, a bundle of seven membrane embedded alpha-helices, binds retinal, a photoreactive chromophore, in a central pocket. In addition to being the pigment of the retina that is responsible for both the formation of the photoreceptor cells, its function is to specifically convey information stored in the specific geometry of the chormophore to the surface of the molecule upon light absorption. In the active state, rhodopsin activates transduction, a GTP binding protein. Once activated, transduction promotes the hydrolysis of cGMP by phosphodiesterase. Rhodopsin’s activity is believed to be shut off by its phosphorylation followed by binding of the soluble protein arrestin. Mutations in the rhodopsin gene lead to retinitis pigmentosa, which can be inherited as an autosomal dominant, an autosomal recessive or an X-linked recessive disorder. Signaling and GTPase research.

Immunogen Type :

Other

Low Endotoxin :

Sample Size :

Application Text :

ELISA,Immunohistochemistry,IF Microscopy,Immunoprecipitation,Western Blot,

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

UniProt :

P02699

NCBI :

NP_001014890.1

Primary Image Name :

Rhodopsin Western Blot

more info or order :

Rockland Immunochemicals product webpage