Piccolo Antibody | Rockland Immunochemicals 200-301-G30 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

Piccolo Antibody

catalog :

200-301-G30

quantity :

100 µg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

6H9-B6

reactivity :

human, rat

application :

western blot, ELISA

more info or order :

Rockland Immunochemicals product webpage

product information

Catalog Number :

200-301-G30

Name :

Anti-Piccolo (MOUSE) Monoclonal Antibody - 200-301-G30

Display Name :

Piccolo Antibody

Application Note :

Anti-Piccolo Antibody is suitable for use in WB, ICC/IF, and ELISA. Expect a band ~550kDa in specific lysate or tissue samples. Specific conditions for reactivity should be optimized by the end user.

Buffer :

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Monoclonal

Clone ID :

6H9-B6

Concentration Value :

1mg/mL

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

(None)

Size :

100 µg

Default Unit :

µg

ELISA Dilution :

1:200

IF Microscopy Dilution :

1:100

Western Blot Dilution :

1:1000

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG2b

Gene Name :

PCLO

Host Animal :

Mouse

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

Piccolo Antibody was produced in mice by repeated immunizations raised against a full length protein.

Packing Type :

Dry Ice

Physical State :

Liquid (sterile filtered)

Purity and Specificity :

Anti-Piccolo Antibody was purified by Protein G chromatography. A BLAST analysis was used to suggest cross-reactivity with Piccolo from human, mouse, rat based on 100% homology with the immunizing sequence. No cross-reactivity to Bassoon. Cross-reactivity with Piccolo from other sources has not been determined. Neuroscience research.

Species Reactivity :

Human, Rat

Stabilizer :

50% (v/v) Glycerol

Storage :

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Subclass :

IgG2b kappa

Synonyms :

ACZ Antibody, Aczonin Antibody, PCLO Antibody, Pico Antibody, presynaptic cytomatrix protein Antibody, protein piccolo, Brain-derived HLMN protein, Multidomain presynaptic cytomatrix protein

Target Species :

Human

Background :

Piccolo, also referred to as Aczonin, is a large protein which consists of an N-terminal Zn2+ finger, several piccolo-bassoon homology domains and C-terminal PDZ and C2 domains. In general it is found together with bassoon, a related huge multi-domain protein of the CAZ (cytoskeletal matric assembled at active zones). Piccolo is a scaffolding protein for proteins involved in endo- and exocytosis of synaptic vesicles. Piccolo has also been shown to interfere with clathrin medicated endocytosis by binding to the F-actin and dynamin binding protein Abp1.

Immunogen Type :

Recombinant Protein

Low Endotoxin :

Sample Size :

Application Text :

ELISA,IF Microscopy,Western Blot,

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

UniProt :

Q9Y6V0

NCBI :

NP_055325.2

Primary Image Name :

Piccolo Western Blot

more info or order :

Rockland Immunochemicals product webpage