p38 alpha MAPKinase Antibody | Rockland Immunochemicals 200-301-G27 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

p38 alpha MAPKinase Antibody

catalog :

200-301-G27

quantity :

100 µg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

9F12

reactivity :

human

application :

western blot, ELISA, immunoprecipitation

more info or order :

Rockland Immunochemicals product webpage

product information

Catalog Number :

200-301-G27

Name :

Anti-p38 alpha MAPKinase (MOUSE) Monoclonal Antibody - 200-301-G27

Display Name :

p38 alpha MAPKinase Antibody

Application Note :

Anti-p38 MAP kinase Antibody is recommended for use in WB, IP, and ELISA. Expect a band approximately ~38kDa protein corresponding to the molecular mass of p38α MAPK on SDS PAGE immunoblots. Specific conditions for reactivity should be optimized by the end user.

Buffer :

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Monoclonal

Clone ID :

9F12

Concentration Value :

1.0 mg/mL

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

(None)

Size :

100 µg

Default Unit :

µg

ELISA Dilution :

1:200

Immunoprecipitation Dilution :

User Optimized

Western Blot Dilution :

1:1000

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG1

Gene Name :

MARK14

Host Animal :

Mouse

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

p38 Antibody was produced in mice by repeated immunizations raised against a full length recombinant protein expressed in E.coli cells.

Packing Type :

Dry Ice

Physical State :

Liquid (sterile filtered)

Purity and Specificity :

Anti- p38 α MAP Kinase Antibody was purified by Protein G chromatography. A BLAST analysis was used to suggest no known cross-reactivity with other related human kinases. Cross-reactivity with p38α MAP kinase from other sources has not been determined. Cell Signaling research.

Species Reactivity :

Human

Stabilizer :

50% (v/v) Glycerol

Storage :

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Subclass :

IgG1

Synonyms :

CSAID Binding protein 1, CSBP1, CSBP2, EXIP, MAP kinase MXI2, MAPkinase p38alpha, p38 ALPHA, p38 MAP kinase, p38 mitogen activated protein kinase, RK, SAPK 2A, Stress activated protein kinase 2A, Mitogen-activated protein kinase 14, Cytokine suppressive anti-inflammatory drug-binding protein

Target Species :

Human

Background :

The MAPK (mitogen activated protein kinase) comprises a family of ubiquitous praline-directed, proteinserine/threonine kinases which signal transduction pathways that control intracellular events including acute responses to hormones and major developmental changes in organisms. This super family consists of stress activated protein kinases (SAPKs); extracellular signal-regulated kinases (ERKs); and p38 kinases, each of which forms a separate pathway. The kinase members that populate each pathway are sequentially activated by phosphorylation. Upon activation, p38 MAPK/SAPK2α translocates into the nucleus where it phosphorylates one or more nuclear substrates, effecting transcriptional changes and other cellular processes involved in cell growth, division, differentiation, inflammation, and death. Specifically p38 always acts as a pro-apoptotic factor with its activation leading to the release of cytochrome c from mitochondria and cleavage of caspase 3 and its downstream effector, PARP. p38 MAPK is activated by a variety of chemical stress inducers including hydrogen peroxide, heavy metals, anisomycin, sodium salicylate, LPS, and biological stress signals such as tumor necrosis factor, interleukin-1, ionizing and UV irradiation, hyperosmotic stress and chemotherapeutic drugs. As a result, p38 alpha has been widely validated as a target for inflammatory disease including rheumatoid arthritis, COPD and psoriasis and has also been implicated in cancer, CNS and diabetes.

Immunogen Type :

Recombinant Protein

Low Endotoxin :

Sample Size :

Application Text :

ELISA,Immunoprecipitation,Western Blot,

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

UniProt :

Q16539

NCBI :

NP_001306.1

Primary Image Name :

p38 alpha MAPKinase Western Blot

more info or order :

Rockland Immunochemicals product webpage