LAMP1 Antibody | Rockland Immunochemicals 200-301-G09 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

LAMP1 Antibody

catalog :

200-301-G09

quantity :

100 µg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

Ly1C6

reactivity :

rat

application :

western blot, immunohistochemistry, immunoprecipitation

more info or order :

Rockland Immunochemicals product webpage

product information

Catalog Number :

200-301-G09

Name :

Anti-LAMP1 (MOUSE) Monoclonal Antibody - 200-301-G09

Display Name :

LAMP1 Antibody

Application Note :

Anti-LAMP1 Antibody is suitable for WB, IP, and IF microscopy. Expect a band approximately ~120kDa protein corresponding to the molecular mass of LAMP1 on SDS PAGE immunoblots. Specific conditions for reactivity should be optimized by the end user.

Buffer :

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Monoclonal

Clone ID :

Ly1C6

Concentration Value :

1 mg/mL

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

(None)

Size :

100 µg

Default Unit :

µg

Immunohistochemistry Dilution :

User Optimized

Immunoprecipitation Dilution :

User Optimized

Western Blot Dilution :

1µg/ml

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG1

Gene Name :

Lamp1

Host Animal :

Mouse

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

LAMP1 Antibody was produced in mice by repeated immunizations raised against rat liver lysosomal membrane preparations.

Packing Type :

Dry Ice

Physical State :

Liquid (sterile filtered)

Purity and Specificity :

Anti-LAMP1 Antibody was purified by Protein G chromatography. A BLAST analysis was used to suggest cross-reactivity with LAMP1 from Hamster and Rat based on 100% homology with the immunizing sequence. Cross-reactivity with LAMP1 from other sources has not been determined.

Species Reactivity :

Rat, Hamster

Stabilizer :

50% (v/v) Glycerol

Storage :

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Subclass :

IgG1

Synonyms :

CD107, CD107a, LAMPA, LGP120, IgpA, Lysosome-associated membrane glycoprotein 1, CD107 antigen-like family member A, 120 kDa lysosomal membrane glycoprotein, Lamp-1

Target Species :

Rat

Background :

Lysosme associated membrane proteins, or LAMP1 and LAMP2, are major constituents of the lysosomal membrane. The two have closely related structures, with 37% sequence homology. They are both transmembrane glycoproteins that are localized primarily in lysosomes and late endosomes. Newly synthesized molecules are mostly transported from the trans-Golgi network directly to endosomes and then to lysosomes. A second pathway involves the lamps being delivered from the Golgi to the cell surface, and then along the endocytic pathway to the lysosomes. A minor pathway involves transport via the plasma membrane. Upon stimulation, a rapid translocation of intracellular LAMPs to the cell membrane is dependent on a carboxylterminal tyrosine based motif (YXXI). If there is a disturbance in this spacing, lysosome localization of LAMP1 is abolished and the mutant protein then cycles between the membrane and the endosome. This stimulation has also been shown to have an associated release of histamine, leukotriene C and prostaglandin D, which shows that LAMP-1 and LAMP-2 are activation markers for normal mast cells. They have also been linked to the inflammatory response in that they promote adhesion of human peripheral blood mononuclear cells (PBMC) to vascular endothelium, and therefore possibly the adhesion of PBMC to the site of inflammation. Ideal for investigators involved in cytokines, growth factors and cancer, as well as, Chaperones and Organelle Markers research.

Immunogen Type :

Other

Low Endotoxin :

Sample Size :

Application Text :

Immunohistochemistry,Immunoprecipitation,Western Blot,

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

UniProt :

P14562

NCBI :

NP_036989.1

Primary Image Name :

LAMP1 Immunofluorescence

more info or order :

Rockland Immunochemicals product webpage