HSP40 (HDJ1) Antibody | Rockland Immunochemicals 200-301-F56 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

HSP40 (HDJ1) Antibody

catalog :

200-301-F56

quantity :

100 µg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

3B9.E6

reactivity :

human, mouse, rat

application :

western blot, ELISA, immunohistochemistry, immunoprecipitation

more info or order :

Rockland Immunochemicals product webpage

product information

Catalog Number :

200-301-F56

Name :

Anti-HSP40 (HDJ1) (MOUSE) Monoclonal Antibody - 200-301-F56

Display Name :

HSP40 (HDJ1) Antibody

Application Note :

Anti-Hsp40 Antibody is recommended for use in WB, IP, IHC, IF, and ELISA. Expect a band approximately ~40kDa protein corresponding to the molecular mass of Hsp40 on SDS PAGE immunoblots. Specific conditions for reactivity should be optimized by the end user.

Buffer :

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Monoclonal

Clone ID :

3B9.E6

Concentration Value :

1mg/mL

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

(None)

Size :

100 µg

Default Unit :

µg

ELISA Dilution :

1:200

Immunohistochemistry Dilution :

User Optimized

IF Microscopy Dilution :

10ug/ml

Immunoprecipitation Dilution :

User Optimized

Western Blot Dilution :

1:2000

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG1

Gene Name :

DNAJB1

Host Animal :

Mouse

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

Hsp40 Antibody was produced in mice by repeated immunizations raised against recombinant Protein Hsp40 (Hdj1).

Packing Type :

Dry Ice

Physical State :

Liquid (sterile filtered)

Purity and Specificity :

Anti-Hsp40 Antibody was purified by Protein G chromatography. A BLAST analysis was used to suggest cross-reactivity with Hsp40 from Human, Mouse, and Rat based on 100% homology with the immunizing sequence. Does not cross-react with HDJ1 or YDJ1. Cross-reactivity with Hsp40 from other sources has not been determined. Heat Shock research.

Species Reactivity :

Human, Mouse, Rat

Stabilizer :

50% (v/v) Glycerol

Storage :

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Subclass :

IgG1

Synonyms :

DNAJ1, NDAJB1, HDJ1, Hsp40, HSPF1, DnaJ homolog subfamily B member 1, Dna J protein homolog 1, Heat shock 40 kDa protein 1, HSP40, heat shock protein 40, Human DnaJ protein 1, hDj-1

Target Species :

Human

Background :

Human Hsp40/DnaJ proteins comprise a large protein family, members of which feature the J domain (named after the bacterial DnaJ protein). The J-domain spans the first 75 N-terminal amino acids and is separated from the C-terminal by a glycine/phenylalanine-rich domain. Members of the Hsp40/DnaJ family play diverse roles in many cellular processes, such as folding, translocation, degradation and assembly of multi-protein complexes. In particular, Hdj1, the first human Hsp40/DnaJ protein identified, plays an important role in protein translation and folding, as well as in the regulation of Hsp70 function. HSP40 stimulates the ATPase activity of HSP70 which in turn causes conformational changes of the unfolded proteins. The Hsp40-Hsp70-unfolded protein complex further binds to co-chaperones Hip, Hop and HSP90 which leads to protein folding, or components of protein degradation machinery CHIP and BAG-1. Some studies have shown that the difference between HDJ1 and type 1 DNAJ proteins including HDJ2 and yeast YdjI is the result of the possession of a zinc finger domain by the latter, which helps in the function of protein folding.

Immunogen Type :

Recombinant Protein

Low Endotoxin :

Sample Size :

Application Text :

ELISA,Immunohistochemistry,IF Microscopy,Immunoprecipitation,Western Blot,

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

UniProt :

P25685

NCBI :

NP_006136.1

Primary Image Name :

Hsp40 (Hdj1) Immunofluorescence

more info or order :

Rockland Immunochemicals product webpage