HSP22 Antibody | Rockland Immunochemicals 200-301-F53 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

HSP22 Antibody

catalog :

200-301-F53

quantity :

100 µg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

3C12-H11

reactivity :

human, mouse, rat

application :

western blot, ELISA, immunohistochemistry

more info or order :

Rockland Immunochemicals product webpage

product information

Catalog Number :

200-301-F53

Name :

Anti-HSP22 (MOUSE) Monoclonal Antibody - 200-301-F53

Display Name :

HSP22 Antibody

Application Note :

Anti-Hsp22 Antibody is suitable for use in WB, IHC and ELISA. Specific conditions for reactivity should be optimized by the end user.

Buffer :

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Monoclonal

Clone ID :

3C12-H11

Concentration Value :

1mg/mL

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

(None)

Size :

100 µg

Default Unit :

µg

ELISA Dilution :

1:200

Immunohistochemistry Dilution :

User Optimized

Western Blot Dilution :

1:2000

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG1

Gene Name :

HSPB8

Host Animal :

Mouse

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

Hsp22 Antibody was produced in mice by repeated immunizations raised against a his-tagged human recombinant hsp22.

Packing Type :

Dry Ice

Physical State :

Liquid (sterile filtered)

Purity and Specificity :

Anti-Hsp22 Antibody was purified by Protein G chromatography. A BLAST analysis was used to suggest cross-reactivity with Hsp22 from human, mouse, and rat based on 100% homology with the immunizing sequence. Detects endogenous and exogenous hsp22 in monomeric, dimeric and tetrameric forms in WB. Does not cross react with alpha crystallin. Cross-reactivity with Hsp22 from other sources has not been determined. Heat Shock research.

Species Reactivity :

Human, Mouse, Rat

Stabilizer :

50% (v/v) Glycerol

Storage :

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Subclass :

IgG1 kappa

Synonyms :

Alpha crystallin C chain, CMT2L, CRYAC, DHMN2, H11, Heat shock 22kDa protein 8, HMN2, HSB8, HspB8, Heat shock protein beta-8, E2IG1, HSP22, E2-induced gene 1 protein, Protein kinase H11

Target Species :

Human

Background :

Hsp22 (HSPB8) is a 196-amino acid protein that is a member of the small heat shock protein super-family and the human protein is most closely related to Hsp27. Similar to most other sHSPs, Hsp22 is predominately transcribed in skeletal muscle and heart, as well as the placenta. Hsp22 is a monomeric protein which interacts with HSPB1. It displays temperature-dependent chaperone activity. In a two hybrid screen, HspB8 interacted preferentially with a triple aspartate form of Hsp27 which mimics Hsp27 phosphorylated at Ser15, Ser78, and Ser82, as compared to wild-type Hsp27. HSPB8 has two binding domains (N and C Terminal) that are specific for different binding partners, and has the ability to bind itself and other sHSPs (3). The chaperone-like activity is of great importance to the function of Hsp22 in various processes including proliferation, apoptosis and macroautophagy. Mutations in the HSPB8 gene are associated with the inherited peripheral neurpathies, autosomal dominant distal hereditary motor neuropathy type IIA (dSMA) and axonal Charcot-Marie-Tooth disease type 2L (CMT2L).

Immunogen Type :

Recombinant Protein

Low Endotoxin :

Sample Size :

Application Text :

ELISA,Immunohistochemistry,Western Blot,

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

UniProt :

Q9UJY1

NCBI :

NP_055180.1

Primary Image Name :

Hsp22 Immunohistochemistry

more info or order :

Rockland Immunochemicals product webpage