HIF-1-alpha Antibody | Rockland Immunochemicals 200-301-F45 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

HIF-1-alpha Antibody

catalog :

200-301-F45

quantity :

100 µg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

ESEE122

reactivity :

human, mouse, rat, bovine

application :

western blot, immunohistochemistry

more info or order :

Rockland Immunochemicals product webpage

product information

Catalog Number :

200-301-F45

Name :

Anti-HIF1 alpha (MOUSE) Monoclonal Antibody - 200-301-F45

Display Name :

HIF-1-alpha Antibody

Application Note :

Anti-HIF1α Antibody is recommended for use in IF microscopy, IHC , WB (weak), and ELISA. Specific conditions for reactivity should be optimized by the end user.

Buffer :

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Monoclonal

Clone ID :

ESEE122

Concentration Value :

1.0 mg/mL

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

(None)

Size :

100 µg

Default Unit :

µg

Immunohistochemistry Dilution :

1:1000-1:8000

IF Microscopy Dilution :

8-21ug/mL

Western Blot Dilution :

User Optimized

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG1

Gene Name :

HIF1A

Host Animal :

Mouse

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

HIF1 alpha Antibody was produced in mice by repeated immunizations raised against a recombinant fragment corresponding to an internal region of HIF1 alpha.

Packing Type :

Dry Ice

Physical State :

Liquid (sterile filtered)

Preservative :

0.09% (w/v) Sodium Azide

Purity and Specificity :

Anti-HIF1α Antibody was purified by Protein G chromatography. A BLAST analysis was used to suggest cross-reactivity with HIF1α from human, mouse, rat, and cow based on 100% homology with the immunizing sequence. Cross-reactivity with HIF1α from other sources has not been determined. Oxidative Stress/Cell Signaling research.

Species Reactivity :

Human, Mouse, Rat, Bovine

Stabilizer :

50% (v/v) Glycerol

Storage :

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Subclass :

IgG1

Synonyms :

ARNT interacting protein, HIF1A, Hypoxia inducible factor 1 alpha, MOP1, PASD8,

Target Species :

Human

Background :

Hypoxia-inducible factor 1 (HIF1) is a heterodimeric transcription factor that plays a critical role in the cellular response of hypoxia. The HIF1 complex consists of two subunits, HIF1-Alpha and HIF1-Beta, which are basic helix-loop-helix proteins of the PAS family. HIF1 regulates the transcription of a broad range of genes that facilitate responses to the hypoxic environment, including genes regulating angiogenesis, erythropoiesis, cell cycle, metabolism and apoptosis. The widely expressed HIF-1α is typically degraded rapidly in normoxic cells by the ubiquitin/proteasomal pathway. Under normoxic conditions, HIF-1α is proline hydroxylated leading to a conformational change that promotes binding to the von Hippel Lindau protein (VLH) E3 ligase complex; ubiquitination and proteasomal degradation follows. Both hypoxic conditions and chemical hydroxylase inhibitors (such as desferrioxamine and cobalt) inhibit HIF-1α degradation and lead to its stabilization. In addition, HIF-1α can be induced in an oxygen-independent manner by various cytokines through the PI3K-AKT-mTOR pathway.

Immunogen Type :

Recombinant Protein

Low Endotoxin :

Sample Size :

Target Domain of Reactivity :

Internal

Application Text :

Immunohistochemistry,IF Microscopy,Western Blot,

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

UniProt :

Q16665

NCBI :

4504385

Primary Image Name :

HIF1 alpha Immunohistochemistry

more info or order :

Rockland Immunochemicals product webpage