GRP78 Antibody | Rockland Immunochemicals 200-301-F36 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

GRP78 Antibody

catalog :

200-301-F36

quantity :

100 µg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

1H11-1H7

reactivity :

human, mouse, rat, bovine

application :

western blot

more info or order :

Rockland Immunochemicals product webpage

product information

Catalog Number :

200-301-F36

Name :

Anti-GRP78 (MOUSE) Monoclonal Antibody - 200-301-F36

Display Name :

GRP78 Antibody

Application Note :

Anti-GRP78 Antibody is suitable for use in WB. Expect a band approximately ~78kDa corresponding to specific lysates. Specific conditions for reactivity should be optimized by the end user.

Buffer :

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Monoclonal

Clone ID :

1H11-1H7

Concentration Value :

1mg/mL

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

(None)

Size :

100 µg

Default Unit :

µg

Western Blot Dilution :

1:1000

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG2b

Gene Name :

HSPA5

Host Animal :

Mouse

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

Grp78 Antibody was produced in mice by repeated immunizations with a his-tagged human GRP78.

Packing Type :

Dry Ice

Physical State :

Liquid (sterile filtered)

Preservative :

0.09% (w/v) Sodium Azide

Purity and Specificity :

Anti-GRP78 Antibody was purified by Protein G chromatography. A BLAST analysis was used to suggest cross-reactivity with GRP78 from Human, Mouse, Rat, Cow, Cryphonectria parasitica, Hamster, Monkey, Rabbit, and Xenopus based on 100% homology with the immunizing sequence. Cross-reactivity with GRP78 from other sources has not been determined. Heat Shock research.

Species Reactivity :

Human, Mouse, Rat, Bovine, Cryphonectria parasitica, Hamster, Monkey, Rabbit, Xenopus

Stabilizer :

50% (v/v) Glycerol

Storage :

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Subclass :

IgG2b

Synonyms :

BiP, Grp78, HspA5, MIF2, immunoglobulin heavy chain binding protein, 78 kDa glucose-regulated protein, Heat shock 70 kDa protein 5

Target Species :

Human

Background :

GRP78 is a ubiquitously expressed, 78-kDa glucose- regulated protein, and is commonly referred to as an immunoglobin chain binding protein (BiP). The BiP proteins are categorized as stress response proteins because they play an important role in the proper folding and assembly of nascent protein and in the scavenging of misfolded proteins in the endoplasmic reticulum lumen. Translation of BiP is directed by an internal ribosomal entry site (IRES) in the 5' nontranslated region of the BiP mRNA. BiP IRES activity increases when cells are heat stressed. GRP78 is also critical for maintenance of cell homeostasis and the prevention of apoptosis. Luo et al. have provided findings that suggest GRP78 is essential for embryonic cell growth and pluripotent cell survival. In terms of diseases, GRP78 has been shown to be a reliable biomarker of hypoglycemia, to serve a neuroprotective function in neurons exposed to glutamate and oxidative stress, and its protein levels are reduced in the brains of Alzheimer’s patients. Also, the induction of the GRP78 protein that results in severe glucose and oxygen deprivation could possible lead to drug resistance to anti-tumor drugs.

Immunogen Type :

Recombinant Protein

Low Endotoxin :

Sample Size :

Application Text :

Western Blot,

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

UniProt :

P11021

NCBI :

NP_005338.1

Primary Image Name :

GRP78 Western Blot

more info or order :

Rockland Immunochemicals product webpage