Grp75 Antibody | Rockland Immunochemicals 200-301-F35 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

Grp75 Antibody

catalog :

200-301-F35

quantity :

100 µg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

S52A-42

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunoprecipitation

more info or order :

Rockland Immunochemicals product webpage

product information

Catalog Number :

200-301-F35

Name :

Anti-Grp75 (MOUSE) Monoclonal Antibody - 200-301-F35

Display Name :

Grp75 Antibody

Application Note :

Anti-Grp75 Antibody is suitable for WB, IP, and IHC. Expect a band approximately 75kDa protein, corresponding to the molecular mass of Grp75 on SDS Page immunoblots. Specific conditions for reactivity should be optimized by the end user.

Buffer :

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Monoclonal

Clone ID :

S52A-42

Concentration Value :

1mg/mL

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

(None)

Size :

100 µg

Default Unit :

µg

Immunohistochemistry Dilution :

User Optimized

Immunoprecipitation Dilution :

User Optimized

Western Blot Dilution :

1:1000

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG1

Gene Name :

HSPA9

Host Animal :

Mouse

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

Grp75 Antibody was produced in mice by repeated immunizations with a synthetic peptide immunogen corresponding to SALM2.

Packing Type :

Dry Ice

Physical State :

Liquid (sterile filtered)

Preservative :

0.09% (w/v) Sodium Azide

Purity and Specificity :

Anti-Grp75 Antibody was purified by Protein G chromatography. A BLAST analysis was used to suggest cross-reactivity with Grp75 from Human, Mouse, and Rat based on 100% homology with the immunizing sequence. Cross-reactivity with Grp75 from other sources has not been determined. Heat Shock research.

Species Reactivity :

Human, Mouse, Rat

Stabilizer :

50% (v/v) Glycerol

Storage :

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Subclass :

IgG1

Synonyms :

Hsc74, Hsp74, HspA9, Hspa9a, HspA9B, Mortalin 2, MOT2, PBP74, ss-70 protein, mitochondrial, 75 kDa glucose-regulated protein, GRP-75, Heat shock 70 kDa protein 9, Mortalin, MOT, Peptide-binding protein 74, PBP74

Target Species :

Human

Background :

Grp75, also known as mortalin, is a member of Hsp70 family of chaperone proteins that is not heat inducible. Grp75 is actually induced under conditions of low glucose and other nutritional and environmental stresses. Grp75 resides primarily in the mitochondrial matrix, where it collaborates with Hsp60 in the re-folding of proteins translocated into this organelle. Related forms may also be found in the cytosol or on the surface of the extracellular membrane. Other Grp75 functions include its ability to inactivate the tumor suppressor p53. Studies have found that Grp75 is over-expressed in many tumor tissues and immortalized human cell lines, suggesting its role in the tumor formation. Grp75 is also implicated in cell aging, as its overexpression appears to prolong the life span of human fibroblasts. And finally, like its E.coli homolog DnaK, GRP75 possesses a cation-dependent ATPase activity considered central to its function as a chaperone.

Immunogen Type :

Peptide

Low Endotoxin :

Sample Size :

Target Domain of Reactivity :

Internal

Application Text :

Immunohistochemistry,Immunoprecipitation,Western Blot,

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

UniProt :

P38646

NCBI :

NP_004125.3

Primary Image Name :

Grp75 Western Blot

more info or order :

Rockland Immunochemicals product webpage