FKBP51 Antibody | Rockland Immunochemicals 200-301-F28 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

FKBP51 Antibody

catalog :

200-301-F28

quantity :

100 µg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

Hi51B

reactivity :

human, mouse, rat, dogs

application :

western blot

more info or order :

Rockland Immunochemicals product webpage

product information

Catalog Number :

200-301-F28

Name :

Anti-FKBP51 (MOUSE) Monoclonal Antibody - 200-301-F28

Display Name :

FKBP51 Antibody

Application Note :

Anti-FKBP51 Antibody is suitable for IF and WB. Expect a band approximately ~51kDa protein representing FKBP51 in cell lysates. Specific conditions for reactivity should be optimized by the end user.

Buffer :

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Monoclonal

Clone ID :

Hi51B

Concentration Value :

1.0 mg/mL

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

(None)

Size :

100 µg

Default Unit :

µg

IF Microscopy Dilution :

10 µg/mL

Western Blot Dilution :

1:2000

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG

Gene Name :

FKBP5

Host Animal :

Mouse

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

FKBP51 Antibody was produced in mice by repeated immunizations with a synthetic peptide corresponding to the residues of human FKBP51.

Packing Type :

Dry Ice

Physical State :

Liquid (sterile filtered)

Preservative :

0.09% (w/v) Sodium Azide

Purity and Specificity :

Anti-FKBP51 Antibody was purified by Protein G chromatography. A BLAST analysis was used to suggest cross-reactivity with FKBP51 from Canine, Hamster, Human, Mouse, Rat, and Rabbit based on 100% homology with the immunizing sequence. Also detects FKBP51 in whole tissue extracts from rat kidney and rat and mouse testes. Cross-reactivity with FKBP51 from other sources has not been determined. Heat Shock research.

Species Reactivity :

Canine, Hamster, Human, Mouse, Rat, Rabbit

Stabilizer :

50% (v/v) Glycerol

Storage :

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Subclass :

IgG1

Synonyms :

AIG6, FK506 binding protein 5, FKBP5, FKBP54, FKBP51, Hsp90 binding immunophilin, p54, Pplase, Ptg10, Rotamase, T cell FK506 binding protein antibody

Target Species :

Human

Background :

Hsp90 is crucial to cellular signaling by its regulation of the folding, activity, and stability of a wide range of client proteins. These client protein complexes may also contain one or more cochaperones. One class of Hsp90-binding cochaperone is composed of proteins with a characteristic tetratricopeptide repeat (TPR) domain that forms an Hsp90 binding site. Among the TPR cochaperones of Hsp90 are Hop/Sti1, protein phosphatase PP5, and members of both the FK506- and cyclosporin A-binding families of immunophilins. FK506-binding protein 51 (FKBP51) and FKBP52 are large molecular weight immunophilins that are part of the mature glucocorticoid receptor (GR) heterocomplex. The N terminal domain of each protein binds FK506 and has peptidyl-prolyl isomerase (PPIase) activity that converts prolyl peptide bonds within target proteins from cis- to trans- proline. The C-terminal domains contain the TPR repeats involved in protein-protein interactions with the Hsp90. Although FKBP52 and FKBP51 share ~75% sequence similarity, they affect hormone binding by glucocorticoid receptor in opposing manners and have different Hsp90-binding characteristics . FK506 binding protein 51 kDa (FKBP51 or otherwise referred to as FKBP54) has been identified as a progestininducible gene. This protein is predominantly expressed in murine T cells but in humans, it is abundantly expressed in numerous tissues at levels many times higher than FKBP12. The FKBP51 gene is known to be induced by glucocorticoids.

Immunogen Type :

Peptide

Low Endotoxin :

Sample Size :

Target Domain of Reactivity :

Internal

Application Text :

IF Microscopy,Western Blot,

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

UniProt :

Q13451

NCBI :

NP_001139247.1

Primary Image Name :

FKBP51 Immunofluorescence

more info or order :

Rockland Immunochemicals product webpage