Alpha B Crystallin Antibody | Rockland Immunochemicals 200-301-E85 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

Alpha B Crystallin Antibody

catalog :

200-301-E85

quantity :

200 µg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

1A7.D5

reactivity :

human, bovine

application :

western blot, ELISA

more info or order :

Rockland Immunochemicals product webpage

product information

Catalog Number :

200-301-E85

Name :

Anti-Alpha B Crystallin (MOUSE) Monoclonal Antibody - 200-301-E85

Display Name :

Alpha B Crystallin Antibody

Application Note :

Anti-Alpha B Crystallin Antibody is recommended for use in WB and ELISA. Expect a band approximately ~20kDa (Predicted mol. weight is ~21kDa) corresponding to αB-crystallin. Specific conditions for reactivity should be optimized by the end user.

Buffer :

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Monoclonal

Clone ID :

1A7.D5

Concentration Value :

1mg/mL

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

(None)

Size :

200 µg

Default Unit :

µg

ELISA Dilution :

1:200

Western Blot Dilution :

1:2000

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG1

Gene Name :

CRYAB

Host Animal :

Mouse

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

Alpha B Crystallin Antibody was produced in mice by repeated immunizations with Native Alpha B Crystallin protein.

Packing Type :

Dry Ice

Physical State :

Liquid (sterile filtered)

Purity and Specificity :

Anti-Alpha B Crystallin Antibody was purified by Protein G chromatography. A BLAST analysis was used to suggest cross-reactivity with alpha B crystallin from Human and bovine based on 100% homology with the immunizing sequence. Does not cross-react with αA-crystallin, βL-crystallin, βH-crystallin, γ-crystallin, Hsp25, Hsp27 or Hsp47 proteins. Cross-reactivity with alpha B crystallin from other sources has not been determined. Heat Shock research.

Species Reactivity :

Human, Bovine

Stabilizer :

50% (v/v) Glycerol

Storage :

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Subclass :

IgG1

Synonyms :

AACRYA, CRYA2, CRYAB, CTPP2, HspB5, NY Ren 27 antigen antibody, alpha crystallin B antibody, Heat shock protein beta-5, Renal carcinoma antigen NY-REN-27, Rosenthal fiber component

Target Species :

Human

Background :

The alpha-crystallins are major water-soluble lens structural proteins of the vertebrate eye that are related to the small heat shock protein family. The alpha-crystallins possess structural and functional similarities with Hsp25 and Hsp27. Mammalian lens cystallins are divided into alpha, beta and gamma families. Alpha and beta families are further divided into acidic and basic groups (Alpha-A and Alpha-B respectively). In the lens, alpha-crystallin primarily functions to maintain proper refractive index, however it can also function as a molecular chaperone that binds to the denatured proteins, keeping them in solution and thereby maintaining the translucency of the lens. When cellular stress occurs, alpha-crystallin enters its’ phosphorylated state and may serve a structural control function and play a role in protein maintenance. In addition to their interaction with proteins, alpha-crystallins also interact with native molecules such as membrane proteins, Golgi matrix protein, structural proteins, nuclear proteins and DNA. Two other functions are an autokinase activity and participation in the intracellular architecture, and it has also been proven that both alpha-A and B prevent apoptosis by inhibiting caspases. Specifically, alpha-B cystallin is found in many cells and organs outside the lens, and alpha B is overexpressed in several neurological disorders and in cell lines under stress conditions.

Immunogen Type :

Native Protein

Low Endotoxin :

Sample Size :

Application Text :

ELISA,Western Blot,

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

UniProt :

P02511

NCBI :

NP_001876.1

Primary Image Name :

Alpha B Crystallin Western Blot

more info or order :

Rockland Immunochemicals product webpage