Rhodopsin Antibody | Rockland Immunochemicals 200-301-E29 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

Rhodopsin Antibody

catalog :

200-301-E29

quantity :

100 µL

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

1D4

reactivity :

bovine

application :

western blot, immunohistochemistry

more info or order :

Rockland Immunochemicals product webpage

product information

Catalog Number :

200-301-E29

Name :

Anti-Rhodopsin (Mouse) Monoclonal Antibody - 200-301-E29

Display Name :

Rhodopsin Antibody

Application Note :

Anti-Rhodopsin antibody is suitable for use in Western Blot and Immunohistochemistry. Expect a band of approximately 39 kDa in size corresponding to the rhodopsin proteins in Western blot in the appropriate cell lysate or extract. Researchers should determine optimal titers for applications that are not stated below.

Buffer :

0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5

Clonality :

Monoclonal

Clone ID :

1D4

Concentration Value :

Titrated value sufficient to run approximately 10 mini blots.

Conjugation :

(None)

Size :

100 µL

Default Unit :

Immunohistochemistry Dilution :

1:100

Western Blot Dilution :

1:1000

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG1

Gene Name :

RHO

Host Animal :

Mouse

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

Anti-Rhodopsin Monoclonal Antibody was produced in mouse by repeated immunizations with purified native bovine rhodopsin.

Packing Type :

Dry Ice

Physical State :

Liquid

Purity and Specificity :

Anti-Rhodopsin antibody is directed against bovine Rhodopsin. Rhodopsin antibodies are Protein G purified from cell culture supernantant. Reactivity is predicted to human and mouse based on strong homolgy. Cross reactivity from other sources has not been determined.

Species Reactivity :

amphibians, most mammals

Stabilizer :

0.1 mg/ml Bovine Serum Albumin (BSA) - IgG and Protease free, 50% (v/v) Glycerol

Storage :

Store vial at -20° C prior to opening. This product is stable at 4° C as an undiluted liquid. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Subclass :

IgG1

Synonyms :

OPN2, Rhodopsin, RHO, Anti-Rhodopsin monoclonal, photoreceptor proteins

Target Species :

Bovine

Background :

Rhodopsin antibody detects rhodopsin which is a photoreceptor protein found in retinal rods. It is a complex formed by the binding of retinaldehyde, the oxidized form of retinol, to the protein opsin and undergoes a series of complex reactions in response to visible light resulting in the transmission of nerve impulses to the brain. Mutation of the rhodopsin gene is a major contributor to various retinopathies such as retinitis pigmentosa. The disease-causing protein generally aggregates with ubiquitin in inclusion bodies, disrupts the intermediate filament network and impairs the ability of the cell to degrade non-functioning proteins which leads to photoreceptor apoptosis. Other mutations on rhodopsin lead to X-linked congenital stationary night blindness, mainly due to constitutive activation, when the mutations occur around the chromophore binding pocket of rhodopsin. Several other pathological states relating to rhodopsin have been discovered including poor post-Golgi trafficking, dysregulative activation, rod outer segment instability and arrestin binding. Anti-Rhodopsin Antibody is ideal for investigators involved in Cell Signaling, Neuroscience, Signal Transduction research.

Immunogen Type :

Native Protein

Low Endotoxin :

Sample Size :

Application Text :

Immunohistochemistry,Western Blot,

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

UniProt :

P02699

Primary Image Name :

Immunohistochemical staining of Anti-Rhodopsin (Mouse) Antibody - 200-301-E29

more info or order :

Rockland Immunochemicals product webpage