product summary
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company name :
Rockland Immunochemicals
product type :
antibody
product name :
FTO Antibody
catalog :
200-301-D41
quantity :
100 µL
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
5-2H10
reactivity :
human, mouse, rat
application :
western blot
product information
Catalog Number :
200-301-D41
Name :
Anti-FTO (Fat mass and obesity related protein) (Mouse) Monoclonal Antibody - 200-301-D41
Display Name :
FTO Antibody
Application Note :
Anti-FTO (Mouse) Antibody is suitable for use in Western Blotting and IF. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 58 kDa in size corresponding to FTO proteins in Western blots of rat testes lysate. This antibody has also been used for immunocytochemistry with neuronal progenitor cells.
Buffer :
0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5
Clonality :
Monoclonal
Clone ID :
5-2H10
Concentration Value :
Titrated value sufficient to run approximately 10 mini blots.
Conjugation :
(None)
Size :
100 µL
Default Unit :
ul
IF Microscopy Dilution :
1:100
Western Blot Dilution :
1:1000
Expiration :
Expiration date is one (1) year from date of opening.
Format :
IgG
Gene Name :
FTO
Host Animal :
Mouse
General Disclaimer Note :
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Immunogen :
Anti-FTO Monoclonal Antibody was produced in mouse by repeated immunizations with FTO- Synthetic peptide corresponding to amino acid residues from the N-terminal region conjugated to KLH.
Packing Type :
Dry Ice
Physical State :
Liquid
Purity and Specificity :
Anti-FTO antibody is directed against human Fat mass and Obesity Related Protein. Anti-FTO is a Protein G purified antibody from cell culture supernatant. Reactivity is expected with the following species based on 100% sequence homology: bovine, canine, horse, non-human primate, sheep.
Species Reactivity :
human, mouse, rat
Stabilizer :
0.1 mg/ml Bovine Serum Albumin (BSA) - IgG and Protease free, 50% (v/v) Glycerol
Storage :
Store vial at -20° C prior to opening.  This product is stable at 4° C as an undiluted liquid.  For extended storage, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. Dilute only prior to immediate use.
Subclass :
IgG
Synonyms :
Alpha-ketoglutarate-dependent dioxygenase FTO, KIAA1752
Target Species :
Human
Background :
FTO Antibody detects human FTO which is the protein encoded by FTO gene that is the most robust gene for common obesity characterized to date. FTO gene expression has been found to be significantly upregulated in the hypothalamus of rats after food deprivation and strongly negatively correlated with the expression of orexin peptide which is involved in the stimulation of food intake. Deletion analysis of FTO gene in mice showed that FTO is functionally involved in the control of both energy intake and energy expenditure. Anti-FTO Antibody is ideal for investigators involved in Neuroscience and Cell Metabolism.
Immunogen Type :
Peptide
Low Endotoxin :
No
Sample Size :
No
Target Domain of Reactivity :
N-Terminus
Application Text :
IF Microscopy,Western Blot,
Category :
Primary Antibodies
Conjugation Name :
Unconjugated
UniProt :
Q9C0B1
NCBI :
AAH30798.1
Primary Image Name :
Western Blot of Anti-FTO (Fat mass and obesity related protein) (Mouse) Antibody - 200-301-D41
company information
Rockland Immunochemicals
321 Jones Blvd
Pottstown, PA 19464
tech@rockland.com
https://www.rockland.com/
484-791-3823
headquarters: USA
Rockland Immunochemicals, Inc. produces Phosho-Site Specific Antibodies and Antibody based tools for basic, applied and clinical research. Our laboratory is located west of Philadelphia, Pennsylvania, USA. The technology base of the organization is the experience of its staff scientists in the production and purification of a wide range of antibodies through monoclonal & polyclonal antibody techniques utilizing in vitro and in vivo methods. Rockland's antibodies are suited for individuals performing Western Blotting, ELISA, Immunohistochemistry, Fluorescent Microscopy, High Content Screening and diagnostic kit production. Primary antibodies include Akt Pathway, Apolipoproteins, Apoptosis/Cell Cycle, Cytokines, Cell Signaling, Enzymes, Extracellular Matrix, Transcription Factors (NFkB) and Ubiquitin.