LIN28 Antibody [1G9H9] | Rockland Immunochemicals 200-301-CL0 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

LIN28 Antibody [1G9H9]

catalog :

200-301-CL0

quantity :

100 µg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

[1G9H9]

reactivity :

human

application :

western blot, ELISA

more info or order :

Rockland Immunochemicals product webpage

product information

Catalog Number :

200-301-CL0

Name :

Anti-LIN28 (MOUSE) Antibody - 200-301-CL0

Display Name :

LIN28 Antibody [1G9H9]

Application Note :

Anti-Lin28 Antibody has been tested for use in ELISA and Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 23 kDa in Western Blots of specific cell lysates and tissues. Validated in human samples. All other applications and species not yet tested.

Buffer :

0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2

Clonality :

Monoclonal

Clone ID :

[1G9H9]

Concentration Value :

1 mg/mL

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

(None)

Size :

100 µg

Default Unit :

µg

ELISA Dilution :

1:500-1:10,000

Western Blot Dilution :

0.5-1 μg/mL

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG2b

Gene Name :

LIN28A

Host Animal :

Mouse

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

Anti-Lin28 antibody was produced in mice by repeated immunizations with a 15 amino acid peptide near the C-terminus of human Lin28.

Packing Type :

Dry Ice

Physical State :

Liquid (sterile filtered)

Preservative :

0.02% (w/v) Sodium Azide

Purity and Specificity :

Anti-Lin28 Monoclonal Antibody was affinity purified from monospecific antiserum by immunoaffinity chromatography. At least two isoforms of Lin28 are known to exist; this antibody will detect both. Lin28 antibody will not cross-react with Lin28 Homolog B.

Species Reactivity :

Human

Storage :

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Subclass :

IgG2b

Synonyms :

LIN28 Antibody, CSDD1, LIN-28, ZCCHC1, lin-28A

Target Species :

Human

Background :

Lin28 is a transcription factor that was first identified through its key role in the pathway of developmental timing in C. elegans. The role of Lin28 in development suggested that it might be useful in the creation of stem cells that might be beneficial in cell replacement therapies in the treatment of several degenerative diseases. Artificial stem cells, termed induced pluripotent stem (iPS) cells, can be created by expressing Lin28 in addition to the transcription factors POU5F1, Sox2, and NANOG in mouse fibroblasts. More recently, experiments have demonstrated that iPS cells could be generated using expression plasmids expressing Lin28, Sox2, POU5F1 and c-Myc, eliminating the need for virus introduction, thereby addressing a safety concern for potential use of iPS cells in regenerative medicine.

Immunogen Type :

Peptide

Low Endotoxin :

Sample Size :

Target Domain of Reactivity :

C-Terminus

Application Text :

ELISA,Western Blot,

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

UniProt :

Q9H9Z2

NCBI :

EAX07814

Primary Image Name :

Western Blot of Lin28 Antibody

more info or order :

Rockland Immunochemicals product webpage