KLF4 Antibody [4E5C3] | Rockland Immunochemicals 200-301-CE9 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

KLF4 Antibody [4E5C3]

catalog :

200-301-CE9

quantity :

100 µg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

[4E5C3]

reactivity :

human, mouse, rat

application :

western blot, ELISA

more info or order :

Rockland Immunochemicals product webpage

product information

Catalog Number :

200-301-CE9

Name :

Anti-KLF4 (MOUSE) Antibody - 200-301-CE9

Display Name :

KLF4 Antibody [4E5C3]

Application Note :

Anti-KLF4 Antibody has been tested for use in ELISA and Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 55 kDa in Western Blots of specific cell lysates and tissues. Validated in mouse samples. All other applications and species not yet tested.

Buffer :

0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2

Clonality :

Monoclonal

Clone ID :

[4E5C3]

Concentration Value :

1.0 mg/ml

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

(None)

Size :

100 µg

Default Unit :

µg

ELISA Dilution :

1:10,000

Western Blot Dilution :

1 μg/mL

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG1

Gene Name :

KLF4

Host Animal :

Mouse

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

Anti-KLF4 antibody was produced in mice by repeated immunizations with a 20 amino acid synthetic peptide near the C-terminus of human KLF4.

Packing Type :

Dry Ice

Physical State :

Liquid (sterile filtered)

Preservative :

0.02% (w/v) Sodium Azide

Purity and Specificity :

Anti-KLF4 Monoclonal Antibody was affinity purified from monospecific antiserum by immunoaffinity chromatography. At least three isoforms of KLF4 are known to exist; this antibody will detect all three. KLF4 antibody will not cross-react with other Kruppel-like family members.

Species Reactivity :

Human, mouse, rat

Storage :

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Subclass :

IgG1

Synonyms :

KLF4 Antibody [4E5C3] , EZF, GKLF

Target Species :

Human

Background :

KLF4 is a transcription factor that functions as both a transcriptional activator and repressor to regulate proliferation and differentiation of multiple cell types. The role of KLF4 in embryonic development suggested that it might be useful in the creation of stem cells that might be useful in cell replacement therapies in the treatment of several degenerative diseases. Artificial stem cells, termed induced pluripotent stem (iPS) cells, can be created by expressing KLF4 and the transcription factors POU5F1, Sox2, and Lin28 along with c-Myc in mouse fibroblasts. More recently, experiments have demonstrated that iPS cells could be generated using expression plasmids expressing KLF4, Sox2, POU5F1 and c-Myc, eliminating the need for virus introduction, thereby addressing a safety concern for potential use of iPS cells in regenerative medicine. KLF4 interacts directly with POU5F1 and Sox2 in iPS and ES cells and activates the target gene NANOG.

Immunogen Type :

Peptide

Low Endotoxin :

Sample Size :

Target Domain of Reactivity :

C-Terminus

Application Text :

ELISA,Western Blot,

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

UniProt :

O43474

NCBI :

NP_001300981.1

Primary Image Name :

Western Blot of KLF4 Antibody

more info or order :

Rockland Immunochemicals product webpage