TRPC6 Antibody | Rockland Immunochemicals 200-301-B59 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

TRPC6 Antibody

catalog :

200-301-B59

quantity :

100 µg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

3F2.H10.F2

reactivity :

human, mouse

application :

western blot, ELISA, immunohistochemistry

more info or order :

Rockland Immunochemicals product webpage

citations: 1

Reference
Alonso GonzÃ¡lez P, Cabo R, San JosÃ© I, Gago A, Suazo I, GarcÃa SuÃ¡rez O, et al. Human Digital Meissner Corpuscles Display Immunoreactivity for the Multifunctional Ion Channels Trpc6 and Trpv4. Anat Rec (Hoboken). 2017;300:1022-1031 pubmed publisher

product information

Catalog Number :

200-301-B59

Name :

Anti-TRPC6 (MOUSE) Monoclonal Antibody - 200-301-B59

Display Name :

TRPC6 Antibody

Application Note :

This monoclonal antibody is suitable for ELISA, immunohistochemistry and western blotting. Expect a band approximately 106 kDa in size corresponding to TRPC6 protein by western blotting in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user. Use formalin-fixed paraffin-embedded sections for immunohistochemistry. No pre-treatment of sample is required. Strong staining was observed in adrenal, Purkinje neurons, cortical neurons, heart, ganglion cells, renal tubules, Sertoli cells, hepatocytes, skeletal muscle, exocrine pancreas, and germinal centers of lymphoid follicles. Moderate staining was observed in colon epithelium and plasma cells, B-lymphocytes, and parafollicular cells of the thyroid. Faint staining was seen in respiratory epithelium. Prostate and placenta were negative for staining. The antibody produced minimal to no background staining and appeared very specific at 2.5 µg/mL.

Buffer :

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Monoclonal

Clone ID :

3F2.H10.F2

Concentration Value :

1.0 mg/mL

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

(None)

Size :

100 µg

Default Unit :

µg

ELISA Dilution :

1:10,000 - 1:50,000

Immunohistochemistry Dilution :

2.5 µg/mL

Western Blot Dilution :

1:500- 1:2,000

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG1

Gene Name :

TRPC6

Host Animal :

Mouse

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

This monoclonal antibody was produced by repeated immunizations with a synthetic peptide corresponding to a region near the carboxy terminus of human TRPC6 protein.

Packing Type :

Dry Ice

Physical State :

Liquid (sterile filtered)

Preservative :

0.01% (w/v) Sodium Azide

Purity and Specificity :

This product was purified from concentrated tissue culture supernate by Protein A chromatography. This antibody is specific for human TRPC6 protein. A BLAST analysis was used to suggest cross-reactivity with TRPC6 from chimpanzee based on 100% homology with the immunizing sequence. Cross-reactivity with TRPC6 from other sources has not been determined.

Species Reactivity :

human, mouse

Storage :

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Subclass :

IgG1 kappa

Synonyms :

mouse anti-TRPC6 Antibody, TRPC 6, TRP6, short transient receptor potential channel 6 and transient receptor potential cation channel subfamily C member 6

Target Species :

Human

Background :

TRPC6, also known as TRP6, short transient receptor potential channel 6 and transient receptor potential cation channel subfamily C member 6, is thought to form a receptor-activated non-selective calcium permeant cation channel. TRPC6 is probably operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. It is activated by diacylglycerol (DAG) in a membrane-delimited fashion, independently of protein kinase C and may not to be activated by intracellular calcium store depletion. Defects in this gene are a cause of focal segmental glomerulosclerosis (FSGS). Expression of this protein has been reported in tissues such as placenta, lung, spleen, ovary, small intestine, and renal podocytes. Immunohistochemistry studies using polyclonal antibodies to this target have shown moderate to strong staining in cell types such as neurons, breast, respiratory, squamous and prostate epithelium, epidermis, placental trophoblasts, dendritic cells, and subsets of immune cells, and faint to moderate staining of adrenal, colon, ganglion cells, hepatocytes, heart, and testis.

Immunogen Type :

Peptide

Low Endotoxin :

Sample Size :

Target Domain of Reactivity :

C-Terminus

Application Text :

ELISA,Immunohistochemistry,Western Blot,

Other :

User Optimized

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

UniProt :

Q9Y210

NCBI :

5730102

Primary Image Name :

Anti-TRPC6 Antibody - Immunohistochemistry

more info or order :

Rockland Immunochemicals product webpage