8-Hydroxy Guanine Antibody | Rockland Immunochemicals 200-301-A99 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

8-Hydroxy Guanine Antibody

catalog :

200-301-A99

quantity :

100 µg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

15A3

reactivity :

mouse

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry

more info or order :

Rockland Immunochemicals product webpage

citations: 2

Published Application/Species/Sample/Dilution	Reference
western blot; mouse; fig 6b	Jabir M, Hopkins L, Ritchie N, Ullah I, Bayes H, Li D, et al. Mitochondrial damage contributes to Pseudomonas aeruginosa activation of the inflammasome and is downregulated by autophagy. Autophagy. 2015;11:166-82 pubmed publisher
	Maya Mendoza A, Ostrakova J, Kosar M, Hall A, Duskova P, Mistrik M, et al. Myc and Ras oncogenes engage different energy metabolism programs and evoke distinct patterns of oxidative and DNA replication stress. Mol Oncol. 2015;9:601-16 pubmed publisher

product information

Catalog Number :

200-301-A99

Name :

Anti-8-Hydroxy Guanine (MOUSE) Monoclonal Antibody - 200-301-A99

Display Name :

8-Hydroxy Guanine Antibody

Application Note :

This Protein G purified antibody has been tested for use in immunohistochemistry, ICC/IF, Dot Blot, IP, Flow Cytometry, and ELISA. Specific conditions for reactivity should be optimized by the end user.

Buffer :

0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Monoclonal

Clone ID :

15A3

Concentration Value :

1.0 mg/mL

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

(None)

Size :

100 µg

Default Unit :

µg

ELISA Dilution :

User Optimized

Flow Cytometry Dilution :

User Optimized

Immunohistochemistry Dilution :

1:1000

IF Microscopy Dilution :

User Optimized

Immunoprecipitation Dilution :

User Optimized

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG2b

Host Animal :

Mouse

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

This Protein G purified monoclonal antibody was prepared using conventional hybridoma technology after repeated immunizations with 8-hydroxy-guanosine-BSA and casein conjugates.

Packing Type :

Dry Ice

Physical State :

Liquid (sterile filtered)

Preservative :

0.1% (w/v) Sodium Azide

Purity and Specificity :

This Protein G purified Anti-8-Hydroxy Guanine monoclonal antibody recognizes markers of oxidative damage to DNA (8-hydroxy-2'-deoxyguanosine, 8-hydroxyguanine and 8-hydroxyguanosine).

Species Reactivity :

rat, human, mouse

Stabilizer :

50% (v/v) Glycerol

Storage :

Store Anti-8-Hydroxy Guanine antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Subclass :

IgG2b

Synonyms :

8 hydroxy 2’ deoxyguanosine antibody, 8 hydroxyguanine antibody, 8 hydroxyguanosine antibody, 8 OHG antibody, 8-OHG antibody, 8OG antibody, 8OHdG antibody, 8OHG antibody, 8-Hydroxy Guanine Antibody, 8-OH-dG Antibody, DNA/RNA Damage Antibody

Background :

DNA or RNA damage is due to environmental factors and normal metabolic processes inside the cell, that then hinder the ability of the cell to carry out its functions. There are four main types of DNA damage due to endogenous cellular processes: oxidation, alkylation, hydrolysis and mismatch of the bases. During the oxidation of bases, highly reactive chemical entities collectively known as RONS may develop. RONS stands for reactive oxygen and nitrogen species and includes nitric oxide, superoxide, hydroxyl radical, hydrogen peroxide and peroxynitrite. Numerous studies have shown that RONS cause a variety of other issues in addition to DNA damage. 8-hydroxyguanine, 8-hydroxy-2’-deoxyguanosine and 8-hydroxyguanosine are all RNA and DNA markers of oxidative damage. 8-hydroxy-2’-guanosine is produced by reactive oxygen and nitrogen species including hydroxyl radical and peroxynitrite. Specifically its high biological relevance is due to its ability to induce G to T transversions, which is one of the most frequent somatic mutations (2). 8-hydroxy-guanine has been the most frequently studied type of DNA base damage, with studies in diabetes, and cancer. Base modifications of this type arise from radical-induced hydroxylation and cleavage reactions of the purine ring. Finally, 8-hydroxy-guanosine, like 8-hydroxy-2’-guanosine, induces a mutagenic transversion of G to T in DNA. Its role has been tested specifically in the development of diabetes, hypertension and strokes.

Immunogen Type :

Native Protein

Low Endotoxin :

Sample Size :

Application Text :

ELISA,Flow Cytometry,Immunohistochemistry,IF Microscopy,Immunoprecipitation,

Other :

User Optimized

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

Primary Image Name :

8-hydroxy-guanine

more info or order :

Rockland Immunochemicals product webpage