antibody

Nitrotyrosine Antibody

200-301-A98

100 µg

monoclonal

mouse

nonconjugated

39B6

western blot, ELISA, immunohistochemistry

Anti-Nitrotyrosine (MOUSE) Monoclonal Antibody - 200-301-A98

Nitrotyrosine Antibody

This Protein G purified antibody has been tested for use in ELISA, immunoprecipitation, immunohistochemistry and western blotting.  Specific conditions for reactivity should be optimized by the end user.  Approximately 0.7 µg of antibody is sufficient to detect 5 µg of SIN-1 treated BSA by Western blotting using HRP Gt-a-Mouse IgG (p/n 610-103-121) and chemiluminescent substrate (p/n PICOMAX-110).

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Monoclonal

39B6

1.0 mg/mL

(None)

100 µg

µg

1:10,000 - 1:50,000

1:200 - 1:1,000

1:500 - 1:2,000

Expiration date is one (1) year from date of opening.

IgG2a

Mouse

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

This Protein G purified monoclonal antibody was prepared using conventional hybridoma technology after repeated immunizations with 3-(4-Hydroxy-3-nitrophenyl acetamido) propionic acid - BSA conjugate.

Dry Ice

Liquid (sterile filtered)

0.1% (w/v) Sodium Azide

This Protein G purified monoclonal antibody reacts with proteins containing 3-nitrotyrosine moieties and does not detect non-nitrated tyrosine residues.  This antibody is known to react with nitrated tyrosine residues from human, mouse, rat and dog sources.  Reactivity with nitrotyrosine from other sources is also expected.

Human, Mouse, Rat, Dog

50% (v/v) Glycerol

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

IgG2a

Protein tyrosine nitration results in a post-translational modification that is increasingly receiving attention as an important component of nitric oxide signaling (2). While multiple nonenzymatic mechanisms are known to be capable of producing nitrated tyrosine residues, most tyrosine nitration events involve catalysis by metalloproteins such as myeloperoxidase, eosinophilperoxidase (3), myoglobin, the cytochrome P-450s, superoxide dismutase and prostacyclin synthase. Nitrotyrosine may also serve as a biomarker for the effects of reactive nitrogen oxides, based on tyrosine residues becoming nitrated in proteins at sites of inflammation-induced tissue injury (1). The presence of nitro tyrosine-containing proteins has shown high correlation to disease states such as atherosclerosis, Alzheimer’s disease, Parkinson’s disease and amyotrophic lateral sclerosis (4).

Native Protein

No

No

ELISA,Immunohistochemistry,Western Blot,

User Optimized

Primary Antibodies

Unconjugated