Lysine Acetylated Antibody | Rockland Immunochemicals 200-301-A97 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

Lysine Acetylated Antibody

catalog :

200-301-A97

quantity :

100 µg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

7F8

application :

western blot, ELISA, immunohistochemistry

more info or order :

Rockland Immunochemicals product webpage

product information

Catalog Number :

200-301-A97

Name :

Anti-Lysine Acetylated (AcK) (MOUSE) Monoclonal Antibody - 200-301-A97

Display Name :

Lysine Acetylated Antibody

Application Note :

This Protein G purified antibody has been tested for use in ELISA, immunohistochemistry and western blotting. Specific conditions for reactivity should be optimized by the end user. Approximately 1 µg of antibody is sufficient to detect 20 µg of acetylated chicken erythrocyte histones (sodium butyrate treated) by Western blotting using HRP Gt-a-Mouse IgG (p/n 610-103-121) and chemiluminescent substrate (p/n PICOMAX-110). Antibody provided in PBS pH 7.4.

Buffer :

See application note.

Clonality :

Monoclonal

Clone ID :

7F8

Concentration Value :

1.0 mg/mL

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

(None)

Size :

100 µg

Default Unit :

µg

ELISA Dilution :

User Optimized

Immunohistochemistry Dilution :

1:200 - 1:1,000

Western Blot Dilution :

1:500 - 1:2,000

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG1

Host Animal :

Mouse

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

This Protein G purified monoclonal antibody was prepared using conventional hybridoma technology after repeated immunizations with acetylated keyhole limpet hemocyanin (KLH).

Packing Type :

Dry Ice

Physical State :

Liquid (sterile filtered)

Preservative :

0.09% (w/v) Sodium Azide

Purity and Specificity :

This Protein G purified monoclonal antibody reacts with proteins containing acetylated lysine residues and does not detect non-acetylated lysine residues. This antibody is known to react with acetylated lysine residues from mammalian and avian sources. Reactivity with acetylated lysines from other sources is also expected.

Species Reactivity :

mammalian, avian

Stabilizer :

50% (v/v) Glycerol

Storage :

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Subclass :

IgG1

Synonyms :

acetyl Lysine antibody, Acetylated lysine antibody, Lysine antibody

Background :

Post-translational modifications of proteins play critical roles in the regulation and function of many known biological processes. A common posttranscriptional modification of lysine residues involves acetylation (1). The conserved amino-terminal domains of the four core histones (H2A, H2B, H3 and H4) contain lysines that are acetylated by histone acetyltransferases (HATs) and deacetylated by histone deacetylases (HDACs) (2). Protein posttranslational reversible lysine Nε-acetylation and deacetylation have been recognized as an emerging intracellular signaling mechanism that plays critical roles in regulating gene transcription, cell-cycle progression, apoptosis, DNA repair, and cytoskeletal organization (3). The regulation of protein acetylation status is impaired in the pathologies of cancer and polyglutamine diseases (4). HDACs therefore are promising targets for anticancer drugs currently in development (5).

Immunogen Type :

Native Protein

Low Endotoxin :

Sample Size :

Application Text :

ELISA,Immunohistochemistry,Western Blot,

Other :

User Optimized

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

more info or order :

Rockland Immunochemicals product webpage