product summary
Loading...
company name :
Rockland Immunochemicals
product type :
antibody
product name :
Mesothelin Antibody
catalog :
200-301-A88
quantity :
100
price :
512.00 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
MN-1
reactivity :
human
application :
western blot, immunohistochemistry, flow cytometry
more info or order :
Rockland Immunochemicals product webpage
citations: 14
Published Application/Species/Sample/DilutionReference
  • western blot; human; fig 1c
Awuah P, Bera T, Folivi M, Chertov O, Pastan I. Reduced Shedding of Surface Mesothelin Improves Efficacy of Mesothelin-Targeting Recombinant Immunotoxins. Mol Cancer Ther. 2016;15:1648-55 pubmed publisher
Suurs F, Lorenczewski G, Bailis J, Stienen S, Friedrich M, Lee F, et al. Mesothelin/CD3 half-life extended bispecific T-cell engager molecule shows specific tumor uptake and distributes to mesothelin and CD3 expressing tissues. J Nucl Med. 2021;: pubmed publisher
Inoue S, Tsunoda T, Riku M, Ito H, Inoko A, Murakami H, et al. Diffuse mesothelin expression leads to worse prognosis through enhanced cellular proliferation in colorectal cancer. Oncol Lett. 2020;19:1741-1750 pubmed publisher
Jewell M, Gibson J, Guy C, Hyun J, Du K, Oh S, et al. Single-Cell RNA Sequencing Identifies Yes-Associated Protein 1-Dependent Hepatic Mesothelial Progenitors in Fibrolamellar Carcinoma. Am J Pathol. 2020;190:93-107 pubmed publisher
Oehl K, Kresoja Rakic J, Opitz I, Vrugt B, Weder W, Stahel R, et al. Live-Cell Mesothelioma Biobank to Explore Mechanisms of Tumor Progression. Front Oncol. 2018;8:40 pubmed publisher
Leshem Y, O Brien J, Liu X, Bera T, Terabe M, Berzofsky J, et al. Combining Local Immunotoxins Targeting Mesothelin with CTLA-4 Blockade Synergistically Eradicates Murine Cancer by Promoting Anticancer Immunity. Cancer Immunol Res. 2017;5:685-694 pubmed publisher
Zhang J, Khanna S, Jiang Q, Alewine C, Miettinen M, Pastan I, et al. Efficacy of Anti-mesothelin Immunotoxin RG7787 plus Nab-Paclitaxel against Mesothelioma Patient-Derived Xenografts and Mesothelin as a Biomarker of Tumor Response. Clin Cancer Res. 2017;23:1564-1574 pubmed publisher
Khanna S, Thomas A, Abate Daga D, Zhang J, Morrow B, Steinberg S, et al. Malignant Mesothelioma Effusions Are Infiltrated by CD3+ T Cells Highly Expressing PD-L1 and the PD-L1+ Tumor Cells within These Effusions Are Susceptible to ADCC by the Anti-PD-L1 Antibody Avelumab. J Thorac Oncol. 2016;11:1993-2005 pubmed publisher
Illei P, Alewine C, Zahurak M, Cowan M, Montgomery E, Hassan R, et al. Mesothelin Expression in Advanced Gastroesophageal Cancer Represents a Novel Target for Immunotherapy. Appl Immunohistochem Mol Morphol. 2016;24:246-52 pubmed publisher
Alewine C, Xiang L, Yamori T, Niederfellner G, Bosslet K, Pastan I. Efficacy of RG7787, a next-generation mesothelin-targeted immunotoxin, against triple-negative breast and gastric cancers. Mol Cancer Ther. 2014;13:2653-61 pubmed publisher
Zhang J, Bera T, Liu W, Du X, Alewine C, Hassan R, et al. Megakaryocytic potentiating factor and mature mesothelin stimulate the growth of a lung cancer cell line in the peritoneal cavity of mice. PLoS ONE. 2014;9:e104388 pubmed publisher
Zhang J, Qiu S, Zhang Y, Merino M, Fetsch P, Avital I, et al. Loss of mesothelin expression by mesothelioma cells grown in vitro determines sensitivity to anti-mesothelin immunotoxin SS1P. Anticancer Res. 2012;32:5151-8 pubmed
Feng M, Zhang J, Anver M, Hassan R, Ho M. In vivo imaging of human malignant mesothelioma grown orthotopically in the peritoneal cavity of nude mice. J Cancer. 2011;2:123-31 pubmed
Xiang X, Phung Y, Feng M, Nagashima K, Zhang J, Broaddus V, et al. The development and characterization of a human mesothelioma in vitro 3D model to investigate immunotoxin therapy. PLoS ONE. 2011;6:e14640 pubmed publisher
product information
Catalog Number :
200-301-A88
Name :
Anti-Mesothelin (MOUSE) Monoclonal Antibody - 200-301-A88
Display Name :
Mesothelin Antibody
Application Note :
This antibody has been tested for use in immunohistochemistry, flow cytometry, and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 40 kDa in size corresponding to mature mesothelin by western blotting in the appropriate cell lysate or extract. For immunohistochemistry, archival PEFF human tissues were deparaffinized followed by hydration.  Antigen-retrieval is recommended.  Block tissues with 1% BSA in PBS for 30 min at 23° C.  Antibodies are diluted in 1% BSA and reacted with tissue for 60 min at room temperature.
Buffer :
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Clonality :
Monoclonal
Clone ID :
MN-1
Concentration Value :
1.0 mg/mL
Concentration Definition :
by UV absorbance at 280 nm
Conjugation :
(None)
Size :
100
Default Unit :
µg
ELISA Dilution :
1:10,000 - 1:50,000
Flow Cytometry Dilution :
1:800
Immunohistochemistry Dilution :
1:100
Western Blot Dilution :
1:1,000
Expiration :
Expiration date is one (1) year from date of receipt.
Format :
IgG2a
Gene Name :
MSLN
Host Animal :
Mouse
General Disclaimer Note :
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Immunogen :
This antibody was produced in mesothelin-deficient mice by immunizations with plasmid cDNA encoding human MSLN full length protein followed by a single boost of a recombinant human mesothelin-Fc fusion protein.
Packing Type :
Dry Ice
Physical State :
Liquid (sterile filtered)
Preservative :
0.01% (w/v) Sodium Azide
Purity and Specificity :
This antibody is directed against human mesothelin protein. This product was purified from tissue culture supernatant fluid by Protein A chromatography.  Cross reactivity with homologues from other sources has not been tested.
Species Reactivity :
Human
Tested Applications :
FC; IHC; WB
Storage :
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Subclass :
IgG2a
Synonyms :
mouse anti-Mesothelin Antibody, Mesothelian, MN, MB, Pre-pro-megakaryocyte-potentiating factor, CAK1 antigen
Target Species :
Human
Background :
Anti Mesothelin Antibody recognizes Mesothelin that is a glycosyl-phosphatidylinositol–anchored glycoprotein present on the cell surface of various human solid tumors.  The mesothelin (MSLN) gene encodes a 71-kDa precursor protein that is processed to a 40-kDa glycosylphosphatidylinositol–anchored protein that composes the mature portion and an NH2 terminal 31-kDa fragment called megakaryocyte-potentiating factor that is released from the cell.  Mesothelin is a tumor differentiation antigen present at low levels on a restricted set of normal adult tissues, such as mesothelium, but aberrantly over expressed in mesotheliomas, ovarian, and pancreatic cancers.  The biological functions of mesothelin remain elusive.  A recent study showed that mesothelin binds to MUC16/CA125, and that this interaction mediates cell adhesion, suggesting that there may be an important role for MUC16/CA125 and mesothelin in the metastatic spread of ovarian cancer.
Immunogen Type :
Recombinant Protein
Low Endotoxin :
No
Sample Size :
No
Target Domain of Reactivity :
Extracellular
Application Text :
ELISA,Flow Cytometry,Immunohistochemistry,Western Blot,
Other :
User Optimized
Category :
Primary Antibodies
Conjugation Name :
Unconjugated
2025 Price :
512.00 USD
UniProt :
Q13421
NCBI :
NP_005814
Primary Image Name :
Anti-Mesothelin Antibodies - Immunohistochemistry
more info or order :
Rockland Immunochemicals product webpage
company information
Rockland Immunochemicals
321 Jones Blvd
Pottstown, PA 19464
tech@rockland.com
https://www.rockland.com/
484-791-3823
headquarters: USA
Rockland Immunochemicals, Inc. produces Phosho-Site Specific Antibodies and Antibody based tools for basic, applied and clinical research. Our laboratory is located west of Philadelphia, Pennsylvania, USA. The technology base of the organization is the experience of its staff scientists in the production and purification of a wide range of antibodies through monoclonal & polyclonal antibody techniques utilizing in vitro and in vivo methods. Rockland's antibodies are suited for individuals performing Western Blotting, ELISA, Immunohistochemistry, Fluorescent Microscopy, High Content Screening and diagnostic kit production. Primary antibodies include Akt Pathway, Apolipoproteins, Apoptosis/Cell Cycle, Cytokines, Cell Signaling, Enzymes, Extracellular Matrix, Transcription Factors (NFkB) and Ubiquitin.