Wnt1 Antibody | Rockland Immunochemicals 200-301-A37S product information

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product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

Wnt1 Antibody

catalog :

200-301-A37S

quantity :

25 µL

price :

99.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

13F9

reactivity :

human

application :

western blot, ELISA

product information

Catalog Number :

200-301-A37S

Name :

Anti-Wnt1 (MOUSE) Monoclonal Antibody - 200-301-A37S

Display Name :

Wnt1 Antibody

Application Note :

This affinity purified antibody has been tested for use in western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 41 kDa in size corresponding to Wnt1 by western blotting in the appropriate cell lysate or extract.

Buffer :

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Monoclonal

Clone ID :

13F9

Concentration Value :

1.0 mg/mL

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

(None)

Size :

25 µL

Default Unit :

ELISA Dilution :

1:4,000 - 1:20,000

Western Blot Dilution :

1:175 - 1:250

Expiration :

Expiration date is three (3) months from date of opening.

Format :

IgG1

Gene Name :

WNT1

Host Animal :

Mouse

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

Synthetic peptide corresponding to an internal region of human Wnt1 protein

Packing Type :

Dry Ice

Physical State :

Liquid (sterile filtered)

Preservative :

0.01% (w/v) Sodium Azide

Purity and Specificity :

Anti-Wnt1 antibody purified from tissue culture supernatant by Protein-A chromatography followed by extensive dialysis against the buffer stated above. This antibody reacts with human and mouse Wnt1 protein. A BLAST analysis was used to suggest cross-reactivity with Wnt1 from mouse, human, rat, bovine, dog, macaque, opossum and rat based on a 100% homology with the immunizing sequence. Partial cross-reactivity is expected against chicken Wnt1 based on a 91% sequence homology. Cross-reactivity with Wnt1 from other sources has not been determined.

Species Reactivity :

human

Storage :

Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Subclass :

IgG1 kappa

Synonyms :

mouse anti-WNT1 antibody, INT1, Proto-oncogene protein Wnt-1

Target Species :

Human

Background :

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. Wnt1 (Wingless-type MMTV integration site family member 1) is a member of the WNT gene family. It is highly conserved in evolution and the protein encoded by this gene is known to be 98% identical to mouse Wnt1 protein at the amino acid level. Studies in mouse indicate that the Wnt1 protein functions in the induction of the mesencephalon and cerebellum. This gene was originally considered as a candidate gene for Joubert syndrome, an autosomal recessive disorder with cerebellar hypoplasia as a leading feature. However, further studies suggested that the gene mutations might not have a significant role in Joubert syndrome. Wnt1 is secreted as an extracellular matrix protein.

General Reference :

De Ferrari,G.V. and Moon,R.T. (2006) The ups and downs of Wnt signaling in prevalent neurological disorders. Oncogene 25 (57), 7545-7553. Yook,J.I., Li,X.Y., Ota,I., Hu,C., Kim,H.S., Kim,N.H., Cha,S.Y., Ryu,J.K., Choi,Y.J., Kim,J., Fearon,E.R. and Weiss,S.J. (2006) A Wnt-Axin2-GSK3beta cascade regulates Snail1 activity in breast cancer cells. Nat. Cell Biol. 8 (12), 1398-1406. He,J., Sheng,T., Stelter,A.A., Li,C., Zhang,X., Sinha,M., Luxon,B.A. and Xie,J. (2006) Suppressing Wnt signaling by the hedgehog pathway through sFRP-1. J. Biol. Chem. 281 (47), 35598-35602.

Immunogen Type :

Peptide

Low Endotoxin :

Sample Size :

Yes

Application Text :

ELISA,Western Blot,

Other :

User Optimized

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

2019 Price :

99.00 USD

UniProt :

P04628

NCBI :

139743

Primary Image Name :

Anti-Wnt1 Monoclonal Antibody - Western Blot