GAPDH Antibody | Rockland Immunochemicals 200-301-A33 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

GAPDH Antibody

catalog :

200-301-A33

quantity :

100 µg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

33F2.C8.G6.E3

reactivity :

domestic rabbit

application :

western blot, ELISA, immunohistochemistry, immunoprecipitation

more info or order :

Rockland Immunochemicals product webpage

product information

Catalog Number :

200-301-A33

Name :

Anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (Mouse) Monoclonal Antibody - 200-301-A33

Display Name :

GAPDH Antibody

Application Note :

Anti-GAPDH (Mouse) is suitable for use in Western Blotting. Specific conditions should be optimized by the end user. Expect a band of ~38kDa in size corresponding to Glyceraldehyde 3-Phosphate Dehydrogenase protein by Western blot in the appropriate cell lysate or extract.

Buffer :

0.1 M Tris Chloride, 0.5 M Sodium Chloride, pH 8.0

Clonality :

Monoclonal

Clone ID :

33F2.C8.G6.E3

Concentration Value :

1.0 mg/ml

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

(None)

Size :

100 µg

Default Unit :

µg

ELISA Dilution :

User Optimized

Immunohistochemistry Dilution :

User Optimized

IF Microscopy Dilution :

1:100

Immunoprecipitation Dilution :

User Optimized

Western Blot Dilution :

1:1,000

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgM

Gene Name :

GAPDH

Host Animal :

Mouse

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

Anti-GAPDH Monoclonal Antibody was produced by repeated immunizations in mice with rabbit GAPDH protein.

Packing Type :

Dry Ice

Physical State :

Liquid (sterile filtered)

Preservative :

0.1% (w/v) Sodium Azide

Purity and Specificity :

Anti-GAPDH Monoclonal Antibody is directed against rabbit Glyceraldehyde-3-Phosphate Dehydrogenase. The antibody is a total IgM fraction prepared from ascites fluid by selective precipitation and tandem molecular sieve chromatography followed by extensive dialysis against the buffer stated above. Anti-GAPDH antibodies are expected to react with the following species based on 100% sequence homology: rabbit, human, and mouse. Reactivity against other sources is not known.

Species Reactivity :

rabbit, human, mouse

Storage :

Store vial at -20° C prior to opening. This product is stable at 4° C as an undiluted liquid. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Strain :

Mouse - Mixed

Subclass :

IgM

Synonyms :

mouse anti-GAPDH antibody, Loading Control Antibody, G3PDH, GAPD, Glyceraldehyde-3-phosphate dehydrogenase, Peptidyl-cysteine S-nitrosylase GAPDH

Target Species :

Rabbit

Background :

GAPDH Loading Control Monoclonal Antibody detects Glyceraldehyde-3-Phosphate Dehydrogenase. GAPDH is a metabolic enzyme responsible for catalyzing one step in the glycolytic pathway, the reversible oxidative phosphorylation of glyceraldehyde 3-phosphate. Because GAPDH is a protein expressed in large amounts and which is required at all times for important "house keeping" functions, levels of GAPDH mRNA are often measured and used as standards in studies of mRNA expression. Increasingly, scientists are making use of specific antibodies to GAPDH in comparable studies of levels of protein expression. Apart from a role in glycolysis, GAPDH may have other roles such as in the activation of transcription. GAPDH is reported to bind to a variety of other proteins, including the amyloid precursor protein, mutations in which cause some forms of Alzheimer's disease, and the polyglutamine tracts of Huntingtin, the protein product aberrant forms of which are causative of Huntington's disease. Associations with actin and tubulin have also been reported. The protein may also have a role in the regulation of apoptosis, and interestingly migrates from the cytoplasm into the nucleus when cells become apoptotic. Anti-GAPDH antibody is widely used as a loading control for western blotting experiments, allowing comparison between the level of this protein and others in a cell or tissue and is ideal for Neuroscience, Cell Signaling and Cancer Research.

Immunogen Type :

Native Protein

Low Endotoxin :

Sample Size :

Application Text :

ELISA,Immunohistochemistry,IF Microscopy,Immunoprecipitation,Western Blot,

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

UniProt :

P46406

NCBI :

NP_001075722.1

Primary Image Name :

Immunofluorescence Microscopy of Mouse Anti-GAPDH antibody

more info or order :

Rockland Immunochemicals product webpage