ATM phospho S1981 Antibody | Rockland Immunochemicals 200-301-500 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

ATM phospho S1981 Antibody

catalog :

200-301-500

quantity :

100 µg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

7C10D8

reactivity :

human, mouse

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section

more info or order :

Rockland Immunochemicals product webpage

citations: 11

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - paraffin section; mouse; 1:200; loading ...; fig s6b	Jiang Z, Li H, Schroer S, Voisin V, Ju Y, Pacal M, et al. Hypophosphorylated pRb knock-in mice exhibit hallmarks of aging and vitamin C-preventable diabetes. EMBO J. 2022;41:e106825 pubmed publisher
immunocytochemistry; mouse; fig 2	Liu E, Xu N, O Prey J, Lao L, Joshi S, Long J, et al. Loss of autophagy causes a synthetic lethal deficiency in DNA repair. Proc Natl Acad Sci U S A. 2015;112:773-8 pubmed publisher
immunocytochemistry; mouse; fig 4	Shamma A, Suzuki M, Hayashi N, Kobayashi M, Sasaki N, Nishiuchi T, et al. ATM mediates pRB function to control DNMT1 protein stability and DNA methylation. Mol Cell Biol. 2013;33:3113-24 pubmed publisher
western blot; mouse	Schwab K, Smith G, Dressler G. Arrested spermatogenesis and evidence for DNA damage in PTIP mutant testes. Dev Biol. 2013;373:64-71 pubmed publisher
	Rosina M, Langone F, Giuliani G, Cerquone Perpetuini A, Reggio A, Calderone A, et al. Osteogenic differentiation of skeletal muscle progenitor cells is activated by the DNA damage response. Sci Rep. 2019;9:5447 pubmed publisher
	Emery I, Gopalan A, Wood S, Chow K, Battelli C, George J, et al. Expression and function of ABCG2 and XIAP in glioblastomas. J Neurooncol. 2017;133:47-57 pubmed publisher
	Sinha V, Qin L, Li Y. A p53/ARF-dependent anticancer barrier activates senescence and blocks tumorigenesis without impacting apoptosis. Mol Cancer Res. 2015;13:231-8 pubmed publisher
	Broering T, Alavattam K, Sadreyev R, Ichijima Y, Kato Y, Hasegawa K, et al. BRCA1 establishes DNA damage signaling and pericentric heterochromatin of the X chromosome in male meiosis. J Cell Biol. 2014;205:663-75 pubmed publisher
	Leemput J, Masson C, Bigot K, Errachid A, Dansault A, Provost A, et al. ATM localization and gene expression in the adult mouse eye. Mol Vis. 2009;15:393-416 pubmed
	Bartkova J, Bakkenist C, Rajpert De Meyts E, Skakkebaek N, Sehested M, Lukas J, et al. ATM activation in normal human tissues and testicular cancer. Cell Cycle. 2005;4:838-45 pubmed
	Kitagawa R, Bakkenist C, McKinnon P, Kastan M. Phosphorylation of SMC1 is a critical downstream event in the ATM-NBS1-BRCA1 pathway. Genes Dev. 2004;18:1423-38 pubmed

product information

Catalog Number :

200-301-500

Name :

Anti-ATM Protein Kinase pS1981 (MOUSE) Monoclonal Antibody - 200-301-500

Display Name :

ATM phospho S1981 Antibody

Application Note :

This Anti-ATM Protein Kinase pS1981 (MOUSE) Monoclonal Antibody clone has been optimized for IHC, but may also be used for western blotting, flow cytometry, immunoprecipitation, or immunofluorescence microscopy. Indirect immunoperoxidase staining on formaldehyde-fixed, de-paraffinized tissue sections and/or formalin-fixed cultured cells are recommended when using this antibody as described in Bartkova et al 2005. Product p/n 200-301-400 produced from clone 10H11.E12 has been optimized for WB, IP and IF.

Buffer :

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Monoclonal

Clone ID :

7C10D8

Concentration Value :

1.0 mg/mL

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

(None)

Size :

100 µg

Default Unit :

µg

ELISA Dilution :

1:10,000 - 1:50,000

Flow Cytometry Dilution :

User Optimized

Immunohistochemistry Dilution :

1:100 - 1:500

Western Blot Dilution :

1:500- 1:2,000

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG2a

Gene Name :

ATM

Host Animal :

Mouse

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

This antibody was produced from a synthetic peptide S-L-A-F-E-E-G-Sp-Q-S-T-T-I-S-S corresponding to aa 1974-1988 of human ATM.

Packing Type :

Dry Ice

Physical State :

Liquid (sterile filtered)

Preservative :

0.01% (w/v) Sodium Azide

Purity and Specificity :

This Protein A Purified Mab antibody is directed against human ATM and is useful in determining its presence by immunohistochemistry. This monoclonal anti-ATM antibody recognizes the phosphorylated form of the protein in native and over-expressed proteins found in various tissues and extracts. Reactivity is observed against human and mouse ATM. Cross reactivity with ATM from other mammalian sources has not been tested.

Species Reactivity :

human, mouse

Storage :

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Subclass :

IgG2a

Synonyms :

mouse anti-ATM antibody, mouse anti-ATMpS1981 antibody, mouse anti- ATM pS1981 antibody, DKFZp781A0353 antibody, Human phosphatidylinositol 3 kinase homolog antibody, MGC74674 antibody, Serine protein kinase ATM antibody, T cell prolymphocytic leukemia antibody

Target Species :

Human

Background :

Anti ATM pS1981 Antibody recognizes the product of the ATM gene that is mutated in the hereditary disease ataxia-telangiectasia. ATM codes for a protein kinase that acts as a master regulator of cellular responses to DNA double-strand breaks. ATM is normally inactive and the question of how it is activated in the event of DNA damage (due to ionizing radiation for instance) is central to understanding its function. ATM protein is now shown to be present in undamaged cells as an inactive dimer. Low doses of ionizing radiation, which induce only a few DNA breaks, activate at least half of the total ATM protein present, possibly in response to changes in chromatin structure. The ATM gene encodes a 370-kDa protein that belongs to the phosphoinositide 3-kinase (PI(3)K) superfamily, but which phosphorylates proteins rather than lipids. The 350-amino-acid kinase domain at the carboxy terminus of this large protein is the only segment of ATM with an assigned function. Exposure of cells to IR triggers ATM kinase activity, and this function is required for arrests in G1, S and G2 phases of the cell cycle. Several substrates of the ATM kinase participate in these IR-induced cell-cycle arrests. These include p53, Mdm2 and Chk2 in the G1 checkpoint; Nbs1, Brca1, FancD2 and SMC1 in the transient IR-induced S-phase arrest; and Brca1 and hRad17 in the G2/M checkpoint. See Bakkenist, C. J. & Kastan, M. B. Nature 421, 499-506 (2003) for a complete presentation of this antibody's specificity and utility.

Immunogen Type :

Peptide

Low Endotoxin :

Post Translational Modification :

Phosphorylation

Sample Size :

Application Text :

ELISA,Flow Cytometry,Immunohistochemistry,Western Blot,

Other :

User Optimized

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

UniProt :

Q13315

NCBI :

Q13315.3

Primary Image Name :

Anti-ATM Monoclonal Antibody - Immunohistochemistry

more info or order :

Rockland Immunochemicals product webpage