6X His Tag Antibody | Rockland Immunochemicals 200-301-382S product information

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product summary

company name :

Rockland Immunochemicals

product type :

other

product name :

6X His Tag Antibody

catalog :

200-301-382S

quantity :

25 µL

price :

99.00 USD

product information

Catalog Number :

200-301-382S

Name :

Anti-6X HIS EPITOPE TAG (MOUSE) Monoclonal Antibody - 200-301-382S

Display Name :

6X His Tag Antibody

Application Note :

Anti-6X His is optimally suited for monitoring expression of His-tagged fusion proteins. As such, anti-6X His/6X His can be used to identify fusion proteins that contain the 6X His epitope. The antibody recognizes the His tag fused either to the amino- or carboxy- termini of targeted proteins. This antibody has been tested by ELISA and western blotting against both the immunizing peptide and His-containing recombinant proteins. Although not tested, this antibody is likely functional for immunoprecipitation and immunocytochemistry.

Buffer :

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Monoclonal

Clone ID :

33D10.D2

Concentration Value :

1.0 mg/mL

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

(None)

Size :

25 µL

Default Unit :

ELISA Dilution :

1:20,000 - 1:100,000

Flow Cytometry Dilution :

User Optimized

Immunohistochemistry Dilution :

User Optimized

Immunoprecipitation Dilution :

1:200

Western Blot Dilution :

1:2,000 - 1:10,000

Expiration :

Expiration date is three (3) months from date of opening.

Format :

IgG1

Host Animal :

Mouse

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

This His antibody was produced in mice by repeated immunizations with 6X His epitope tag peptide H-H-H-H-H-H conjugated to KLH using maleimide.

Packing Type :

Dry Ice

Physical State :

Liquid (sterile filtered)

Preservative :

0.01% (w/v) Sodium Azide

Purity and Specificity :

6X His antibody was purified from concentrated tissue culture supernate by Protein A chromatography and is directed against the 6X His motif and is useful in determining its presence in various assays. This polyclonal anti-6X His tag antibody detects over-expressed proteins containing the 6X His epitope tag. To date, this antibody has reacted with all His tagged proteins so far tested. In western blotting of bacterial extracts, the antibody does not cross-react with endogenous proteins. The antibody recognizes the His-tag (His-His-His-His-His-His) fused to either the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.

Storage :

Store 6X His Tag Antibody at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Subclass :

IgG1 kappa

Synonyms :

mouse anti-6X His Tag Antibody, anti-HIS, HIS Antibody, 6X His Tag Antibody, HHHHHH epitope tag antibody

Background :

6X His antibody is an Epitope tag which is a short peptide sequence that is easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.

Epitope Tag Type :

6X HIS

Immunogen Type :

Peptide

Low Endotoxin :

Sample Size :

Yes

Application Text :

ELISA,Flow Cytometry,Immunohistochemistry,Immunoprecipitation,Western Blot,

Other :

User Optimized

Category :

GFP and Epitopes

Conjugation Name :

Unconjugated

2019 Price :

99.00 USD

Primary Image Name :

Anti-6x-His Epitope Tag Monoclonal Antibody - ELISA