p53 Antibody | Rockland Immunochemicals 200-301-174 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

p53 Antibody

catalog :

200-301-174

quantity :

100 µg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

BP53-12

reactivity :

human

application :

western blot, ELISA, immunohistochemistry, immunoprecipitation, chromatin immunoprecipitation

more info or order :

Rockland Immunochemicals product webpage

product information

Catalog Number :

200-301-174

Name :

Anti-p53 (MOUSE) Monoclonal Antibody - 200-301-174

Display Name :

p53 Antibody

Application Note :

This antibody is suitable for ChIP, flow cytometry, immunohistochemistry, Immunofluorescence, immunoblotting and immunoprecipitation. p53 is the most commonly mutated gene in spontaneously occurring human cancers. Mutations arise with an average frequency of 70% but incidence varies from zero in carcinoid lung tumors to 97% in primary melanomas. High concentrations of p53 protein are transiently expressed in human epidermis and superficial dental fibroblasts following mild ultraviolet irradiation. This antibody reacts with an N-terminal epitope of the 53 kD gene product and this epitope is not destroyed by formalin-fixation and routine paraffin embedding. Microwaving is needed for optimal staining.

Buffer :

0.02 M Potassium Phosphate, 0.5 M Sodium Chloride, pH 7.2

Clonality :

Monoclonal

Clone ID :

BP53-12

Concentration Value :

1.0 mg/mL

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

(None)

Size :

100 µg

Default Unit :

µg

ELISA Dilution :

1:2,000 - 1:10,000

ChIP Dilution :

1 µg/µL at 4° o/n

Immunohistochemistry Dilution :

1:50

IF Microscopy Dilution :

1:100-1:500

Immunoprecipitation Dilution :

1:100

Western Blot Dilution :

1:500 - 1:2,000

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG2a

Gene Name :

TP53

Host Animal :

Mouse

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

This protein A purified monoclonal antibody was produced by repeated immunizations with recombinant human p53 protein.

Packing Type :

Dry Ice

Physical State :

Liquid (sterile filtered)

Preservative :

0.01% (w/v) Sodium Azide

Purity and Specificity :

This protein A purified mouse monoclonal antibody reacts specifically with p53 in human tissues and cell lines. The antibody recognizes a 53 kDa band corresponding to p53. Cross reactivity with p53 from other sources has not been determined.

Species Reactivity :

human

Storage :

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Subclass :

IgG2a kappa

Synonyms :

mouse anti-p53 antibody, mouse anti-Tumor Suppressor p53 antibody, Phosphoprotein p53 antibody, TP53 antibody, Transformation related protein 53 antibody, TRP53 antibody, cellular Tumor antigen p53 antibody

Target Species :

Human

Background :

The p53 gene like the Rb gene, is a tumor suppressor gene. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism; its activity stops the formation of tumors. If a person inherits only one functional copy of the p53 gene from their parents, they are pre-disposed to cancer and usually develop several independent tumors in a variety of tissues in early adulthood. This condition is rare, and is known as Li-Fraumeni syndrome. However, mutations in p53 are found in most tumor types, and so contribute to the complex network of molecular events leading to tumor formation. Anti-p53 Antibody is ideal for investigators involved in Signaling Proteins, Cell Cycle Proteins, Apoptosis/Autophagy, Cancer, Cardiovascular Disease, Cell Cycle, Cellular Stress, Inflammation, JNK/SAPK Pathway, Metabolic Disorder, Neurobiology, and p38 Pathway research.

Immunogen Type :

Recombinant Protein

Low Endotoxin :

Sample Size :

Application Text :

ELISA,Chip,Immunohistochemistry,IF Microscopy,Immunoprecipitation,Western Blot,

Other :

User Optimized

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

UniProt :

P04637

NCBI :

23491729

Primary Image Name :

p53 Antibody - Immunofluorescence Microscopy

more info or order :

Rockland Immunochemicals product webpage