p21 WAF1 Antibody | Rockland Immunochemicals 200-301-171 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

p21 WAF1 Antibody

catalog :

200-301-171

quantity :

100 µg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

WA-1

reactivity :

human

application :

western blot, ELISA, immunohistochemistry, immunoprecipitation

more info or order :

Rockland Immunochemicals product webpage

product information

Catalog Number :

200-301-171

Name :

Anti-p21/WAF1 (MOUSE) Monoclonal Antibody - 200-301-171

Display Name :

p21 WAF1 Antibody

Application Note :

This antibody is suitable for immunohistochemistry, ELISA, immunofluorescence, and immunoblotting. The antibody detects a 21 kDa band corresponding to the p21 protein. MCF7 cells or UV treated fibroblasts are suggested as a positive control for immunoblotting and immunohistochemistry. Methanol/acetone fixed tissue is reactive for immunohistochemistry. The antibody also reacts with breast carcinoma on frozen sections (methanol/acetone fixation) and on paraffin sections (formalin fixation and microwave treatment prior to staining). In ELISA this monoclonal antibody is useful either as a capture antibody (attached to the solid phase) or for detection of p21 protein. This antibody immunoprecipitates monomeric p21WAF1 and not p21 complexes with other proteins. High level expression of p21WAF1 requires induction of wild-type p53 expression (e.g. via exposure to DNA damaging agents).

Buffer :

0.02 M Potassium Phosphate, 0.5 M Sodium Chloride, pH 7.2

Clonality :

Monoclonal

Clone ID :

WA-1

Concentration Value :

1.0 mg/mL

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

(None)

Size :

100 µg

Default Unit :

µg

ELISA Dilution :

1:5,000 - 1:20,000

Immunohistochemistry Dilution :

User Optimized

IF Microscopy Dilution :

1:250

Immunoprecipitation Dilution :

1:100

Western Blot Dilution :

1:2,000 - 1:10,000

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG1

Gene Name :

CDKN1A

Host Animal :

Mouse

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

This protein A purified monoclonal antibody was produced by repeated immunizations with recombinant human p21 fusion protein.

Packing Type :

Dry Ice

Physical State :

Liquid (sterile filtered)

Preservative :

0.01% (w/v) Sodium Azide

Purity and Specificity :

This protein A purified mouse monoclonal antibody reacts with human and mammalian p21. The intracellular protein p21 is a 21 kD protein and also known as wild-type p53-activated fragment 1 (WAF1). It is an inhibitor of cyclin-dependent kinases (Cdks) and proliferating-cell nuclear antigen (PCNA). The antibody is highly specific and shows no cross-reactivity with other closely related mitotic inhibitors.

Species Reactivity :

human

Storage :

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Subclass :

IgG1 kappa

Synonyms :

mouse anti-p21/WAF1 antibody, Cyclin-dependent kinase inhibitor 1A (p21, CIP1) antibody, DNA Synthesis Inhibitor antibody, MDA 6 antibody, MDA6 antibody, Melanoma Differentiation Associated Protein 6 antibody

Target Species :

Human

Background :

p21WAF1 is a tumor suppressor gene product critical to maintain proper regulatory control of cell division. p21WAF1 inhibits cyclin/cyclin-dependent kinase (cdk) complexes, causing cell cycle arrest by blocking cell division at the S phase. Various mutations of tumor suppressor genes have been associated with different types of cancers. In these cases, somatic gene therapy with wild-type versions of tumor suppressor genes have been contemplated as anti-cancer therapeutic approaches. p21WAF1 contains p53-binding sites in its promoter and expression of p21WAF1 is induced in WI38 fibroblasts with PMA. Anti-p21WAF1 antibody is useful for researches interested in studying tongue and gastrointestinal cancers, as well as, ubiquitin and cyclin binding proteins.

Immunogen Type :

Recombinant Protein

Low Endotoxin :

Sample Size :

Application Text :

ELISA,Immunohistochemistry,IF Microscopy,Immunoprecipitation,Western Blot,

Other :

User Optimized

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

UniProt :

P38936

NCBI :

NP_510867.1

Primary Image Name :

Anti-Human p21WAF1 Monoclonal Antibody - Western Blot

more info or order :

Rockland Immunochemicals product webpage