SIX3 Antibody | Rockland Immunochemicals 200-201-A26 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

SIX3 Antibody

catalog :

200-201-A26

quantity :

50 µg

clonality :

polyclonal

host :

guinea-pigs

conjugate :

nonconjugated

reactivity :

mouse

application :

western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

Rockland Immunochemicals product webpage

citations: 3

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - frozen section; mouse; 1:500; loading ...; fig 4a	Soleilhavoup C, Travaglio M, Patrick K, Garção P, Boobalan E, Adolfs Y, et al. Nolz1 expression is required in dopaminergic axon guidance and striatal innervation. Nat Commun. 2020;11:3111 pubmed publisher
immunohistochemistry - paraffin section; mouse; 1:200	Madrigal M, Moreno Bravo J, MartÃnez LÃ³pez J, MartÃnez S, Puelles E. Mesencephalic origin of the rostral Substantia nigra pars reticulata. Brain Struct Funct. 2016;221:1403-12 pubmed publisher
	O Brien L, Guo Q, Bahrami Samani E, Park J, Hasso S, Lee Y, et al. Transcriptional regulatory control of mammalian nephron progenitors revealed by multi-factor cistromic analysis and genetic studies. PLoS Genet. 2018;14:e1007181 pubmed publisher

product information

Catalog Number :

200-201-A26

Name :

Anti-Homeobox protein SIX3 (GUINEA PIG) Antibody - 200-201-A26

Display Name :

SIX3 Antibody

Application Note :

This Protein A purified antibody has been tested for use in ELISA, immunofluorescence microscopy and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 37 kDa in size corresponding to Six3 by western blotting in the appropriate cell lysate or extract.

Buffer :

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Polyclonal

Concentration Value :

0.65 mg/mL

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

(None)

Size :

50 µg

Default Unit :

µg

ELISA Dilution :

1:5,000 - 1:25,000

Immunohistochemistry Dilution :

1:250 - 1:500

IF Microscopy Dilution :

1:250 - 1:500

Western Blot Dilution :

1:500 - 1:2,000

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG

Gene Name :

Six3

Host Animal :

Guinea Pig

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

This Protein A purified antibody was prepared from whole guinea pig serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of mouse Six3 protein.

Packing Type :

Dry Ice

Physical State :

Liquid (sterile filtered)

Preservative :

0.01% (w/v) Sodium Azide

Purity and Specificity :

This product was purified by Protein A chromatography from monospecific antiserum. This antibody reacts with mouse Six3. Cross-reactivity with Six3 from other sources has not been determined.

Species Reactivity :

mouse

Storage :

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Synonyms :

guinea pig anti-SIX 3 antibody, Six3, Sine oculis homeobox homolog 3

Target Species :

Mouse

Background :

Six3 (also known as sine oculis homeobox homolog 3) is involved in the development of the visual system and forebrain. Six3 is a nuclear protein that is reported to exist in two forms by alternative splicing of the gene product. Six3 is first expressed at E6.5 of mouse embryonic development around the anterior border. At E8.5, expression is found over the anterior neural plate. At E9.5, it is in the diencephalic part of the ventral forebrain, optic vesicles, olfactory placodes and Rathke’s pouch. In later stages, Six3 is present in hypothalamus, eyes and pituitary.

Immunogen Type :

Peptide

Low Endotoxin :

Sample Size :

Target Domain of Reactivity :

Internal

Application Text :

ELISA,Immunohistochemistry,IF Microscopy,Western Blot,

Other :

User Optimized

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

UniProt :

Q62233

NCBI :

59939908

Primary Image Name :

Immunofluorescence of Guinea Pig Anti-Six 3 Antibody

more info or order :

Rockland Immunochemicals product webpage