Lactate Dehydrogenase Antibody | Rockland Immunochemicals 200-1173-0100 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

Lactate Dehydrogenase Antibody

catalog :

200-1173-0100

quantity :

100 µg

clonality :

polyclonal

host :

domestic goat

conjugate :

nonconjugated

reactivity :

domestic rabbit

application :

western blot, ELISA, immunoprecipitation

more info or order :

Rockland Immunochemicals product webpage

product information

Catalog Number :

200-1173-0100

Name :

Anti-LACTATE DEHYDROGENASE (Rabbit Muscle) (GOAT) Antibody - 200-1173-0100

Display Name :

Lactate Dehydrogenase Antibody

Application Note :

LACTATE DEHYDROGENASE antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 36 kDa in size corresponding to LDH western blotting in the appropriate cell lysate or extract.

Buffer :

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Polyclonal

Concentration Value :

1.0 mg/ml

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

(None)

Size :

100 µg

Default Unit :

µg

ELISA Dilution :

1:2,000 - 1:10,000

IF Microscopy Dilution :

1:200

Immunoprecipitation Dilution :

1:100

Western Blot Dilution :

1:1,000 - 1:5,000

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG

Gene Name :

LDHA

Host Animal :

Goat

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

This antibody was prepared from whole goat serum produced by repeated immunizations with a full length lactate dehydrogenase protein isolated from rabbit muscle.

Packing Type :

Ambient

Physical State :

Lyophilized

Preservative :

0.01% (w/v) Sodium Azide

Purity and Specificity :

Anti-LACTATE DEHYDROGENASE is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Lactate Dehydrogenase [Rabbit Muscle]. BLAST analysis was used to determine that cross reactivity is suggested for both muscle and heart isoforms (LDH-A and LDH-B) from most mammalian species.

Reconstitution Buffer :

Restore with deionized water (or equivalent)

Reconstitution Volume :

100 µL

Species Reactivity :

rabbit, human

Storage :

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Synonyms :

goat anti-Lactate Dehydrogenase Antibody, L-lactate dehydrogenase A chain, LDH-A, LDH muscle subunit, LDH-M

Target Species :

Rabbit

Background :

Lactate dehydrogenase is also known as L-lactate dehydrogenase A chain, LDH-A, LDH muscle subunit and LDH-M. Two isozymes of LDH occur in mammals, LDH-M and LDH-H which come together to form a homotetramer of 36 kDa subunits. Every LDH molecule consists of four subunits, where each subunit is either H or M (based on their electrophoretic properties.) There are, therefore, five LDH isotypes: LDH-1 (4H) - in the heart, LDH-2 (3H1M) - in the reticuloendothelial system, LDH-3 (2H2M) - in the lungs, LDH-4 (1H3M) - in the kidneys and LDH-5 (4M) - in the liver and striated muscle. Usually LDH-2 is the predominant form in the serum. An LDH-1 level higher than the LDH-2 level (a ''flipped pattern'') suggests myocardial infarction (damage to heart tissues releases heart LDH, which is rich in LDH-1, into the bloodstream). LDH complex works to prevent muscle failure and fatigue. LDH can be measured when released during tissue breakdown. LDH appears normally throughout the body, and LDH levels rise with many cancers, so it can be used as a tumor marker but not as a cancer identifier. Anti-Lactate Dehydrogenase Antibody is useful for researcher interested in cancer and metabolism research.

Immunogen Type :

Native Protein

Low Endotoxin :

Sample Size :

Application Text :

ELISA,IF Microscopy,Immunoprecipitation,Western Blot,

Other :

User Optimized

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

UniProt :

P13491

NCBI :

126050

Primary Image Name :

Rockland Lactate Dehydrogenase Antibody-Immunofluorescence

more info or order :

Rockland Immunochemicals product webpage