Luciferase Antibody Fluorescein Conjugated | Rockland Immunochemicals 200-102-150-0100 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

Luciferase Antibody Fluorescein Conjugated

catalog :

200-102-150-0100

quantity :

100 µg

clonality :

polyclonal

host :

domestic goat

conjugate :

FITC

application :

flow cytometry

more info or order :

Rockland Immunochemicals product webpage

product information

Catalog Number :

200-102-150-0100

Name :

Anti-LUCIFERASE (GOAT) Antibody Fluorescein Conjugated - 200-102-150-0100

Display Name :

Luciferase Antibody Fluorescein Conjugated

Application Note :

Anti-Luciferase Fluorescein Conjugated is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Buffer :

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Polyclonal

Concentration Value :

1.0 mg/mL

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

FITC

Size :

100 µg

Default Unit :

µg

Flow Cytometry Dilution :

User Optimized

Fluorochrome Protein Value :

5.6

IF Microscopy Dilution :

User Optimized

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG

Host Animal :

Goat

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

Luciferase (Photinus pyralis (Firefly))

Packing Type :

Ambient

Physical State :

Lyophilized

Preservative :

0.01% (w/v) Sodium Azide

Purity and Specificity :

Anti-Luciferase Fluorescein Conjugated is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum and purified and partially purified Luciferase (Photinus pyralis (Firefly)).

Reconstitution Buffer :

Restore with deionized water (or equivalent)

Reconstitution Volume :

100 µL

Species Reactivity :

Firefly

Stabilizer :

10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Storage :

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Synonyms :

goat anti-Luciferase Antibody FITC Conjugation, Fluorescein Conjugated goat anti-Luciferase Antibody

Background :

Anti luciferase Antibody recognizes luciferase that is commonly used in biological research as a reporter to assess the transcriptional activity in cells that are transfected with a genetic construct containing the luciferase gene under the control of a promoter of interest. Luciferase can also be used to detect the level of cellular ATP in cell viability assays or for kinase activity assays. Additionally proluminescent molecules that are converted to luciferin upon activity of a particular enzyme can be used to detect enzyme activity in coupled or two-step luciferase assays. Such substrates have been used to detect caspase activity and cytochrome P450 activity, among others.

Epitope Tag Type :

LUCIFERASE

Immunogen Type :

Native Protein

Low Endotoxin :

Sample Size :

Application Text :

Flow Cytometry,IF Microscopy,

Other :

User Optimized

Category :

GFP and Epitopes

Conjugation Name :

Fluorescein (FITC)

more info or order :

Rockland Immunochemicals product webpage