antibody

Fetuin Antibody

200-101-456

500 µg

401 USD

polyclonal

goat

nonconjugated

western blot, ELISA

Anti-Fetuin (2-HS Glycoprotein) (GOAT) Antibody - 200-101-456

Fetuin Antibody

This purified polyclonal antibody reacts with human fetuin by ELISA and western blot. Although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation.  A doublet band or slightly lower molecular weight band (see Figure 1, lane 1) may be visible by western blot due to proteolytic processing, variable glycosylation and/or phosphorylation.  Proteolytic processing may include the removal of a 40 amino acid residue bridging peptide from the A and B chains of fetuin in vivo.

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Polyclonal

1.0 mg/mL

by UV absorbance at 280 nm

(None)

500 µg

µg

1:40.000 - 1:200,000

1:10,000 - 1:20,000

Expiration date is one (1) year from date of opening.

IgG

AHSG

Goat

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

This purified antibody was prepared from rabbit serum after repeated immunizations with a recombinant human fetuin (a 2 -HS glycoprotein) processed to remove a 40 amino acid residue bridging peptide resulting in the mature form of the protein.

Ambient

Lyophilized

0.01% (w/v) Sodium Azide

This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum.

Restore with deionized water (or equivalent)

500 µL

human

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

goat anti-Fetuin antibody, 59 kDa bone sialic acid-containing protein antibody, A2HS antibody, Aa2-066 antibody, AHS antibody, Ahsg alpha-2-HS-glycoprotein antibody, AHSG antibody, Alpha 2 Z globulin antibody, Alpha-2-HS-glycoprotein antibody, Ba-alpha-2-glycoprotein, Fetuin-A, PRO2743

Human

Human fetuin (2-Heremans-Schmid-glycoprotein or α 2 -HS-glycoprotein) is a major plasma glycoprotein predominantly synthesized in the liver.  Human fetuin is named after its bovine homolog.  Fetuins are found in most mammals. Human fetuin is a negative acute-phase protein; normal circulating levels in adults (300–600 μg/ml) fall significantly (30–50%) during injury and infection. The biological role of fetuin is unknown, although it has been implicated as an immunomodulator that can participate in stimulation of bacterial phagocytosis by neutrophils and promotion of endocytosis by mouse macrophages. Hepatocytes are the principal cell source of circulating fetuin, but it also is expressed by monocyte/macrophages. Fetuins occur in large amounts in blood and cerebrospinal fluid and accumulate to high concentrations in calcified bone.  The fetuin promoter region has several potential interleukin 6-responsive elements, and its synthesis is down-regulated during injury and inflammation. Fetuin is an acidic glycoprotein with three N-linked and three O-linked oligosaccharide chains, whose terminal sugar residues are rich in sialic acid (N-acetylneuraminic acid), contributing to its net negative charge. A role for fetuin as a carrier of bioactive molecules has been proposed based on observations that it binds and carries Ca2+ ion.  Fetuin is implicated in bone remodeling, immune function and may play a role in tumor progression of certain cell types.

Schäfer C. et al. (2003) The serum protein 2–Heremans-Schmid glycoprotein/fetuin-A is a systemically acting inhibitor of ectopic calcification. J. Clin. Invest. 112:357-366. Leite-Browning, M.L. et al. (2002) Interactions of 2-HS-glycoprotein (fetuin) with MMP-3 and mutine squamous cell carcinoma cells. Intl. J. Onc. 21: 965-971. Nawratil, P. et al. (1996) Limited proteolysis of human 2-HS glycoprotein/Fetuin. J. Biol. Chem 271:49, 31735-31741.

Peptide

No

No

ELISA,Western Blot,

User Optimized

Primary Antibodies

Unconjugated

401 USD

P02765

P02765.1

Anti-Fetuin Antibody - Western Blot